We narrowed to 7,745 results for: sms

1. AiP12255-pAAV-AiE0460m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2255)

   Plasmid

   #164455

   Purpose

   AiE0460m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai133 (TITL-ssAPEX2tm) targeting vector

   Plasmid

   #114432

   Purpose

   Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   ssAPEX2tm
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TREtight

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pαH-S-GSAS-B.1.617.2.v1

   Plasmid

   #182575

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ai167 (TIT2L-ChrimsonR-tdT-ICL-tTA2) targeting vector

   Plasmid

   #114431

   Purpose

   Target a Cre-dependent ChrimsonR-tdTomato cassette and a tTA2 cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   ChrimsonR-tdTomato, tTA2
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector

   Plasmid

   #114435

   Purpose

   Target a Cre-dependent voltage sensor ASAP2s cassette into the mouse TIGRE locus

   Depositor

   Michael Lin, Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   ASAP2s-Kv, tTA2
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Kv2.1 tag

   Expression

   Mutation

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP11496 - pAAV-hDlxI56i-minBG-H2B-nsSYFP2-WPRE3-BGHpA (Alias: CN1496)

   Plasmid

   #164449

   Purpose

   Direct-expressing H2BSYFP2 AAV Virus

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   H2B-SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP1045-pAAV-mscRE10-minBGpromoter-iCre-WPRE-hGHpA

   Plasmid

   #163477

   Purpose

   Direct-expressing iCre AAV Virus. Alias: AiP1045 - pAAV-AiE2010m-minBG-iCre-WPRE-HGHpA

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Beta Globin minimal promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP1046-pAAV-mscRE13-minBGpromoter-iCre-WPRE-hGHpA

   Plasmid

   #163478

   Purpose

   Direct-expressing iCre AAV Virus. Alias: AiP1046 - pAAV-AiE2013m-minBG-iCre-WPRE-HGHpA

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Beta Globin minimal promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector

   Plasmid

   #114429

   Purpose

   Target a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette to the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GFP
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Calb1-T2A-dgCre targeting vector

    Plasmid

    #114438

    Purpose

    Target dgCre into the endogenous mouse Calb1 locus at the stop codon

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    dgCre
    

    Use

    Mouse Targeting

    Tags

    DHFR domain and EGFP

    Expression

    Mutation

    Promoter

    none; utilizes endogenous Calb1 promoter for expr…

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUB_SMFLuc0---MS2

    Plasmid

    #210568

    Purpose

    Expresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpins

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-nonoptimal FLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    UBC human

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUB_SMFLuc100---MS2

    Plasmid

    #210570

    Purpose

    Expresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpins

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-optimal FLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    UBC human

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_SM-FLuc0-pA

    Plasmid

    #210583

    Purpose

    Expresses SM(FLAG)-nonoptimal Firefly luciferase- pA

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-nonoptimal FLuc
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_SM-FLuc100-pA

    Plasmid

    #210584

    Purpose

    Expresses SM(FLAG)-optimal Firefly luciferase - pA

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-optimal FLuc
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

    Plasmid

    #213024

    Purpose

    Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

    Depositor

    Oliver Weichenrieder, Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (238-1061)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7 / Lac

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

    Plasmid

    #213025

    Purpose

    Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (1-1275)
    

    Use

    Tags

    3C-3xFlag (ORF2p)

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

    Plasmid

    #164450

    Purpose

    AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Has Service

    AAV PHP.eB

    Insert

    iCre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBetaGlobin

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pαH-S-GSAS-2P-OMICRON

    Plasmid

    #180593

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge