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EcNusGNTD

Plasmid

#113123

Purpose

Expresses N-terminus of E. coli NusG

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

NusG

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 120-181

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ182

Plasmid

#159723

Purpose

Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

GID4 (VID24 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1755

Plasmid

#139879

Purpose

Expression plasmid for naturally occurring 8xMCE protein (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

Denitrovibrio_acetiphilus_Dacet_0211

Expression

Bacterial

Mutation

Denitrovibrio_acetiphilus_Dacet_0211(41-988)-6xHis

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_STS

Plasmid

#139799

Purpose

expresses biosynthetic enzyme STS

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

STS (STS vitis vinifera, Mustard Weed)

Expression

Bacterial

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFd022.R2.RFP

Plasmid

#137971

Purpose

Expresses Prochlorococcus phage P-SSM2 phage Fd fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd029.R2.RFP

Plasmid

#137972

Purpose

Expresses Prochlorococcus marinus NATL1A Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL1A ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd030.R2.RFP

Plasmid

#137973

Purpose

Expresses Prochlorococcus marinus NATL2A Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL2A ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd031.RFP

Plasmid

#137974

Purpose

Expresses Prochlorococcus marinus MIT9211 Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus MIT9211 ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P7SHb + STS:P8SHb

Plasmid

#139793

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P7SHb
STS:P8SHb

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P7S + STS:P8S

Plasmid

#139794

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P7S
STS:P8S

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pFE-sfGFP

Plasmid

#132998

Purpose

pFE carrying a sfGFP gene

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

sfGFP

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGuide-P1-O1a (EL702)

Plasmid

#140040

Purpose

CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

Depositor

Johan Elf

Article

Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

Insert

barcode P1 and sgRNA lacO1 array

Use

Synthetic Biology

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S + STS:P4S

Plasmid

#139789

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

biosynthetic enzymes 4CL and STS in fusion with coiled-coil segments

Expression

Bacterial

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pME18s-Ix_NPYLR1a

Plasmid

#138716

Purpose

Expresses Ixodes scapularis NPY-like receptor 1a (NPYLR1a) in a mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR1a (LOC8025399 Ixodes scapularis)

Expression

Mammalian

Promoter

SRa

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMM587

Plasmid

#133593

Purpose

Part Plasmid for dCas9, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9

Use

Synthetic Biology

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMM586

Plasmid

#133592

Purpose

Part Plasmid for dCas9, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9

Use

Synthetic Biology

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDK580

Plasmid

#134205

Purpose

Baculovirus expresion of CENP-L and CENP-N

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-L and His-CENP-N

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7007

Plasmid

#136946

Purpose

Malachite green aptamer with stability hairpin, bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pKM692

Plasmid

#134224

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 235-509

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM673

Plasmid

#134222

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 510-end

Expression

Insect

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pZS077

Plasmid

#134235

Purpose

Bacterial expression of CENP-C

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

GST-CENP-C

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMM765

Plasmid

#133602

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM756

Plasmid

#133600

Purpose

Part Plasmid for ERG25 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgERG25

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM729

Plasmid

#133598

Purpose

Part Plasmid for Mxi1-dCas9, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

Mxi1-dCas9

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM767

Plasmid

#133604

Purpose

Part Plasmid for dCas9 NLS, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM585

Plasmid

#133591

Purpose

Part Plasmid for dCas9, Part 3a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM766

Plasmid

#133603

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM768

Plasmid

#133605

Purpose

Part Plasmid for dCas9 NLS, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM769

Plasmid

#133606

Purpose

Part Plasmid for dCas9 NLS, Part 3a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM755

Plasmid

#133599

Purpose

Part Plasmid for ERG11 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgERG11

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM764

Plasmid

#133601

Purpose

Part Plasmid for TEF1 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgTEF1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFE-dabA2

Plasmid

#133001

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

dabA2

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabB2

Plasmid

#133000

Purpose

pFE carrying a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

dabA2

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0626

Plasmid

#129001

Purpose

pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pRPL18B-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0622

Plasmid

#128997

Purpose

pTEF1-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0627

Plasmid

#129002

Purpose

pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pTDH3-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0529

Plasmid

#128986

Purpose

Part plasmid for Zif268DBD-CRY2, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-ZiF268DBD-CRY2

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0554

Plasmid

#128990

Purpose

LEU2 integrtion vector for VP16-CIB1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Leu2 5' homology-pTDH3-SV40NLS-VP16-CIB1-tADH1-Con1-LEU2-Leu2 3' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0658

Plasmid

#129019

Purpose

pREV1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0625

Plasmid

#129000

Purpose

pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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