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POC1484

Plasmid

#213810

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1466

Plasmid

#213754

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Truncated non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1482

Plasmid

#213808

Purpose

LguI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

LguI-associated switch methylase M.XmnI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1483

Plasmid

#213809

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1472

Plasmid

#213760

Purpose

BsaI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Osp807II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1470

Plasmid

#213758

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Truncated non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1465

Plasmid

#213742

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Truncated non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1469

Plasmid

#213757

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Truncated non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1471

Plasmid

#213759

Purpose

BsaI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Osp807II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1473

Plasmid

#213761

Purpose

BpiI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated switch methylase M2.NmeMC58II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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POC1463

Plasmid

#213141

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDR274-gsc2-sgRNA

Plasmid

#184818

Purpose

Used to synthesize gRNA targeting exon 2 of the gsc2 gene

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

gsc2-sgRNA

Use

CRISPR; Template for sgrna synthesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-GFP-he1.1:CFP

Plasmid

#184815

Purpose

Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-GFP-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-GFP

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFVp-gI-BlastR

Plasmid

#207179

Purpose

Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFVp

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (TEVp)

Plasmid

#213407

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

HA-arrestin-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBJ016_MTB_CoChR_KGC_eGFP_ER2

Plasmid

#209277

Purpose

Ubiquitously expresses CoChR in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

CoChR_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ037_MTB_FR-GECO1c

Plasmid

#209279

Purpose

Ubiquitously expresses FR-GECO1c in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF- RPA4 (MSW#716)

Plasmid

#208077

Purpose

Expression of human Replication Protein A 4 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4 (RPA4 Human)

Expression

Bacterial

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pJV0016

Plasmid

#205133

Purpose

Expression of 2xPX-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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