We narrowed to 21,685 results for: pET

1. pcDNA3-JNKsub(TA)DD-YPet-NES

   Plasmid

   #84634

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsub(TA)DD-YPet-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-Fab1 (Francisella tularensis)

   Plasmid

   #61690

   Purpose

   Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

   Depositor

   Michael Johnson

   Article

   Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

   Insert

   enoyl-[acyl-carrier-protein] reductase (NADH)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET15b/GgVcl V1-851 (aka Vh)

   Plasmid

   #46172

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

   Insert

   Vcl 1-851 (VCL Chicken)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 1-851 (aka Vh) head domain

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28A-p53 (1-73) (P12A P13A)

   Plasmid

   #62066

   Purpose

   Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains TAD residues 1-73; P12A, P13A

   Promoter

   T7

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET-CH2 (1192-1318)

   Plasmid

   #99338

   Purpose

   Bacterial expression of mouse CBP CH2 domain

   Depositor

   Peter Wright

   Article

   Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

   Insert

   CBP CH2 (1192-1318) (Crebbp Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-mAIM2-deltaPYD-GST

   Plasmid

   #51534

   Purpose

   Express GST-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Tags

   GST and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-At4CL-PhCHS-MsCHI

   Plasmid

   #73392

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   PhCHS
   MsCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-Vv4CL-CmCHS-MsCHI

   Plasmid

   #73402

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Vv4CL
   CmCHS
   MsCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet RagA-RagC(L91P)

   Plasmid

   #99647

   Purpose

   Bacterial expression of RagA-RagC(L91P)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   L91P

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. HOXA9 (murine) FLAG pET

    Plasmid

    #8494

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    FLAG epitope tag replaces T7 tag in the pET back…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pET151-Vta1(280-330)

    Plasmid

    #87738

    Purpose

    Bacterial expression of the VSL domain of S. cerevisiae Vta1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-279

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-22HT-MSI1(7-103)

    Plasmid

    #60357

    Purpose

    Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-103) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 7-103 of Msi1

    Promoter

    T7

    Available Since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET-DEST42-SAR1-V5-His6

    Plasmid

    #67430

    Purpose

    Expression of hSar1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-Hisx6-Sirt6-S56A

    Plasmid

    #20278

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Serine 56 to Alanine

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-hAIM2-PYD-GST

    Plasmid

    #51531

    Purpose

    Express GST-tagged PYD of hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Tags

    GST and T7

    Expression

    Bacterial

    Mutation

    deletion of amino acids 102-343

    Promoter

    lac operator

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b-ACP (from S. aureus)

    Plasmid

    #63686

    Purpose

    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus

    Depositor

    Michael Johnson

    Insert

    ACP
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET11d-FHis-DND1-Y102A

    Plasmid

    #70082

    Purpose

    Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (DND1 Human)
    

    Tags

    6xHIS and FLAG

    Expression

    Bacterial

    Mutation

    Y102A. Codon optimized for bacterial expression

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-DEST42-ARL6-V5-His6

    Plasmid

    #67422

    Purpose

    Expression of hArl6 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL6 (ARL6 Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. HOXC13(human) GSTfusion-pET

    Plasmid

    #8680

    Depositor

    Corey Largman

    Article

    Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

    Insert

    HOXC13 (HOXC13 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    makes protein by Coomassie blue staining

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only