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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,957 results for: pET

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Showing: 5561 - 5580 of 9957 results

  1. pSLS.540

    Plasmid
    #184959
    Purpose
    Test effect of a1/a2 length on editing fabH using retron recombineering
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    fabH donor G954A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184959-plasmid-map-sequence-id-376675

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  2. pSUMO-HsSyx3

    Plasmid
    #179288
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Publication
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179288-plasmid-map-sequence-id-354924

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  3. pGST-HsSyx-375-792

    Plasmid
    #179292
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site
    Depositor
    Debra Hansen
    Publication
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + GST + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179292-plasmid-map-sequence-id-354918

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  5. 6xHis-MBP-huLb5Cas12a

    Plasmid
    #174684
    Purpose
    Expresses 6xHis-MBP tagged Lb5Cas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huLb5Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174684-plasmid-map-sequence-id-346193

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  6. 6xHis-MBP-huPdCas12a

    Plasmid
    #174682
    Purpose
    Expresses 6xHis-MBP tagged PdCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huPdCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174682-plasmid-map-sequence-id-346188

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  7. 6xHis-MBP-huPcCas12a

    Plasmid
    #174681
    Purpose
    Expresses 6xHis-MBP tagged PcCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huPcCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174681-plasmid-map-sequence-id-346186

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  8. 6xHis-MBP-huCMtCas12a

    Plasmid
    #174680
    Purpose
    Expresses 6xHis-MBP tagged CMtCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huCMtCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174680-plasmid-map-sequence-id-346184

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  12. pZF-ADD-L

    Plasmid
    #127477
    Purpose
    coding sequence for synthetic zinc finger ZF-ADD-L
    Depositor
    Sebastian Maerkl
    Publication
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
    Use
    Cell-free transcription–translation
    Tags
    PDZ cognate peptide ligand (VKESLV)
    Promoter
    T7
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127477-plasmid-map-sequence-id-263301

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Showing: 5561 - 5580 of 9957 results