We narrowed to 7,375 results for: ef1a

1. YX022 (PB)EF1a-LIDARsensor (hygR)

   Plasmid

   #233431

   Purpose

   EF1a-driven LIDAR reporter RNA

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2))cSG-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1a

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a-TBET-T2A-mCHERRY

   Plasmid

   #224296

   Purpose

   Expresses mouse TBET and mCherry under EF1a promoter

   Depositor

   Nikhil Joshi

   Article

   Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

   Insert

   TBET T2A mCherry (Tbx21 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a-ER-KLF2-T2A-mCHERRY

   Plasmid

   #224297

   Purpose

   Expresses estrogen receptor linked KLF2 and mCherry under EF1a promoter

   Depositor

   Nikhil Joshi

   Article

   Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

   Insert

   Estrogen Receptor linked KLF2 T2A mCherry (Klf2 Mouse)
   

   Use

   Retroviral

   Tags

   Estrogen Receptor

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-EF1A-HALO-hLRRK2

   Plasmid

   #229738

   Purpose

   Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-EF1A-mSTING (1-339)

   Plasmid

   #229740

   Purpose

   Expresses mouse STING amino acids 1-339 in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   STING (Sting1 Mouse)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-339

   Promoter

   EF1A

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

   Plasmid

   #229800

   Purpose

   Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIV-EF1a-Il1a-ZsGreen

   Plasmid

   #231987

   Purpose

   Express IL-1alpha-ZsGreen fusion protein

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   IL-1alpha-ZsGreen fusion construct
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-EF1a-HSF1-GFP

   Plasmid

   #227820

   Purpose

   Expresses HSF1 fused to GFP from EF1a promoter

   Depositor

   Jeffrey Gerst

   Article

   Pataki et al bioRxiv 2024.10.05.616786

   Insert

   HSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Tags

   3xHA and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

   Plasmid

   #195567

   Purpose

   PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

   Depositor

   Joanna Wysocka

   Article

   Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

   Insert

   TIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-hU6-2O-crScaffold_EF1a-BFP

    Plasmid

    #224862

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC-hU6-2O-crBFP_EF1a-BFP

    Plasmid

    #224783

    Purpose

    BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC-hU6-2O-crEGFP_EF1a-BFP

    Plasmid

    #224784

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crEGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC-hU6-crBFP_EF1a-BFP

    Plasmid

    #224785

    Purpose

    BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC-hU6-CrEGFP_EF1a-BFP

    Plasmid

    #224786

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-EF1aL-E690Kmut_ABCA3-GFP

    Plasmid

    #188549

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3:GFP (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    amino acid E690->K690 (ABCA3 gene)

    Promoter

    EF1aL

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-EF1aL-W308Rmut_ABCA3-GFP

    Plasmid

    #188550

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3 (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    amino acid W308->R308 (ABCA3 gene)

    Promoter

    EF1aL

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-EF1a-pA (JDW 1222)

    Plasmid

    #224526

    Purpose

    A Gateway compatible 3' entry clone containing rat EF-1a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF-1a polyA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-EF1a-HOXB5-DD-hygro

    Plasmid

    #222582

    Purpose

    PiggyBac transposon plasmid for Shield1-inducible expression of BCL2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a Promoter (MTK 2) (pAN2824)

    Plasmid

    #194225

    Purpose

    Mammalian Toolkit part 2 containing the EF1a promoter

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-ArgiNLS-miRFP670

    Plasmid

    #220600

    Purpose

    Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    miRFP670
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-EF1a-ArgiNLS-mKate2

    Plasmid

    #220599

    Purpose

    Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-EF1a-ArgiNLS-AausFP1

    Plasmid

    #220595

    Purpose

    Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLX-EF1a_NKX2-1_DelNTerm_160-371AA

    Plasmid

    #221487

    Purpose

    NKX2-1 N-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 1-159AA N-terminal domain

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX-EF1a_NKX2-1_DelCTerm_1-223AA

    Plasmid

    #221488

    Purpose

    NKX2-1 C-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 224-371AA C-terminal domain

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLX-EF1a_NKX2-1_HD_160-223AA

    Plasmid

    #221489

    Purpose

    NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 1-159AA N-terminal and 224-371AA C-te…

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EF1a-hP53DD (TET+CTD)

    Plasmid

    #214082

    Purpose

    Mammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    hP53DD (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EF1a-SRRD-V5-APEX2 (in pLEX_307)

    Plasmid

    #214921

    Purpose

    constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EF1a-SRRD-noTag (in pLEX_307)

    Plasmid

    #214919

    Purpose

    constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSmart-EF1alpha-NlaCas7-p65

    Plasmid

    #207394

    Purpose

    Expresses NlaCas7-p65 activator fusion in mammalian cells

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    EF1a-NlaCas7-p65-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSmart-EF1alpha-AcrIC8-NLS

    Plasmid

    #207395

    Purpose

    Expresses AcrIC8 with NLS in mammalian cells

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    EF1a-AcrIC8-bGH polyA
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge