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Plasmid#157774PurposeProduces a fusion between mTurquoise2 and shadowG. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneUseTagsmTurquoise2 and shadowGExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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pMM0638
Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB-CAG-TEVp-IRES-mCherry
Plasmid#174377PurposeFor making TEVP cell line with hypBaseDepositorInsertTEV Protease
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
UseTags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zeb2-3'UTR
Plasmid#61771PurposeLuciferase reporter containing the 3'UTR of mouse Zeb2DepositorInsertmouse Zeb2 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK285 (Stag-3FLAG-Bsr)
Plasmid#72801PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Bsr
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
UseTags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Stra6L-6His
Plasmid#174215PurposeStra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.DepositorInsertStra6L (Stra6l Mouse)
UseTags6-HisExpressionMammalianMutationnonPromoterCMVAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-ADAM29i1
Plasmid#59606PurposeExpression of shRNA against human ADAM29DepositorInsertADAM29 (ADAM29 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hmga2 3'UTR m4 luciferase (Luc-m4)
Plasmid#14787DepositorInsertLuc-m4 (Hmga2 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationm4 mutant (see article and author's map for …PromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMM0641
Plasmid#129006PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2NtB2
Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hmga2 3'UTR m2 luciferase (Luc-m2)
Plasmid#14786DepositorInsertLuc-m2 (Hmga2 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationm2 mutant (see article and author's map for …PromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBabe HcRed Ras
Plasmid#10678DepositorInsertHras V12 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V, constitutively activePromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXR1AtR2B2NtS1
Plasmid#160879PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: pBAD, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA-Thermal_CTLA-4
Plasmid#199751PurposeThermal expression of CTLA-4DepositorInsertCTLA-4 (Ctla4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
-2.5Kb MMP10
Plasmid#53973PurposeLuciferase reporter for mouse MMP10 2.5kb promoterDepositorInsertmouse MMP10 2.5Kb promoter (Mmp10 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterPCR from C57BL6/JAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2B2
Plasmid#160880PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtTcR1AtR2NtB2
Plasmid#160889PurposeExpression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-Tc
UseTagsExpressionBacterialMutationPromoterrbcS-Tc: T7, chaperones: T12Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDI
Plasmid#60555Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT2R1AtR2NtB2
Plasmid#160886PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T2
UseTagsExpressionBacterialMutationPromoterrbcS-T2: T7, chaperones: T9Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDIb′a′
Plasmid#60556Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIb'a' tagExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-mouseCENP-C
Plasmid#174444Purposein vitro transcription template for mouse CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV mTurquoise2-superYFP tandem
Plasmid#157776PurposeProduces a fusion between mTurquoise2 and superYFP. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneUseTagsmTurquoise2 and superYFPExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zfp36-3'UTR
Plasmid#61780PurposeLuciferase reporter containing the 3'UTR of mouse Zfp36DepositorInsertmouse Zfp36 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExodus
Plasmid#39991DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCMV, T7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP
Plasmid#45578PurposeExpresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) neo with Smad5 CDS
Plasmid#61800PurposepcDNA3.1(+) with full length mouse Smad5 CDSDepositorInsertSmad5 CDS (Smad5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGFPmAID_htz-1
Plasmid#174214PurposeDonor plasmid for tagging htz-1 with GFP::mAIDDepositorInsertGFP::mAID::htz-1 tagging donor
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only