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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-Caspase-11_enzymatic-domain

Plasmid

#183382

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-11 (AA100-373) (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-TUBB

Plasmid

#66942

Purpose

CRISPaint target selector TUBB

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA TUBB

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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YH001 CMV-TO-tm-GS+XTEN-ADAR2(DD-E396A)-RXR (FLP-IN)

Plasmid

#233383

Purpose

ER-retained catalytically inactive ADAR2dd (E396A mutation)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

TM-ADAR2dd

Expression

Mammalian

Mutation

E396A

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-ACTG1

Plasmid

#66943

Purpose

CRISPaint target selector ACTG1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA ACTG1

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a RanGAP

Plasmid

#13387

Depositor

Mary Dasso

Article

Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

Insert

RanGAP (RANGAP1 Human)

Expression

Bacterial

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCAS9-mCherry-HIST1H4C

Plasmid

#66944

Purpose

CRISPaint target selector HIST1H4C

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA HIST1H4C

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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a5-ecto-pcDNA31hygro

Plasmid

#113386

Purpose

Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

Insert

human alpha 5 ectodomain

Tags

Strep II tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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PV247

Plasmid

#132338

Purpose

CasA constitutive expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

CasA

Expression

Plant

Mutation

Codon optimize for expression and stability in Ze…

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG387

Plasmid

#105338

Purpose

Synthetic biology; gateway destination vector assembly

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_GY11_00054591

Plasmid

#121338

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

GY11_00054591

Use

Golden gate

Mutation

Magnaporthe oryzae

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFTK066

Plasmid

#171338

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Gal4 AD (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRO275

Plasmid

#233825

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48-1XOaf1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-1XOaf1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO276

Plasmid

#233826

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48-2XOaf1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-2XOaf1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO288

Plasmid

#233827

Purpose

MoClo-YTK; type4A; Activation domains; VP48

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

VP48

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN102

Plasmid

#233828

Purpose

MoClo-YTK; type4A; Activation domains; NLS-VP48-2XOaf1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48-2XOaf1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL002

Plasmid

#233829

Purpose

MoClo-YTK; type4A; Activation domains; VP48-2XOaf1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

VP48-2XOaf1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO129

Plasmid

#233805

Purpose

MoClo-YTK; type3; rtTA systems; rtTA3

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

rtTA3

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO112

Plasmid

#233806

Purpose

MoClo-YTK; type3; rtTA systems; rtTA-v10

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

rtTA-v10

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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