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vYM004_pNeo_phiC31_nTRE_pEF1s(orig)_citrine

Plasmid

#197677

Purpose

"pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM004_pNeo_phiC31_nTRE_pEF1s(orig)_citrine

Plasmid

#197677

Purpose

"pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pC1-ND-Cdt1-mCitrine

Plasmid

#193771

Purpose

Human Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradation

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

ND-Cdt1-mCitrine (CDT1 Human)

Tags

mCitrine

Expression

Mammalian

Mutation

amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pC1-ND-Cdt1-mCitrine

Plasmid

#193771

Purpose

Human Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradation

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

ND-Cdt1-mCitrine (CDT1 Human)

Tags

mCitrine

Expression

Mammalian

Mutation

amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CaMKII-Aion-ts-citrine-ER

Plasmid

#192576

Purpose

AAV-vector for optogenetic long-term silencing of excitatory neurons

Depositor

Simon Wiegert

Insert

Aion

Use

AAV

Tags

ts-Citrine-ER

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

CaMKIIa(0.4)

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CaMKII-Aion-ts-citrine-ER

Plasmid

#192576

Purpose

AAV-vector for optogenetic long-term silencing of excitatory neurons

Depositor

Simon Wiegert

Insert

Aion

Use

AAV

Tags

ts-Citrine-ER

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

CaMKIIa(0.4)

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mDlx-Aion-citrine

Plasmid

#192572

Purpose

AAV-vector for optogenetic long-term silencing of GABAergic neurons

Depositor

Simon Wiegert

Insert

Aion

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

minimal Dlx

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mDlx-Aion-citrine

Plasmid

#192572

Purpose

AAV-vector for optogenetic long-term silencing of GABAergic neurons

Depositor

Simon Wiegert

Insert

Aion

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

minimal Dlx

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-ocitnahC

Plasmid

#163898

Purpose

Luciferase reporter for mouse Cxcl1 with Chantico promoter in reverse orientation

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Promoter

Cxcl1

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-ocitnahC

Plasmid

#163898

Purpose

Luciferase reporter for mouse Cxcl1 with Chantico promoter in reverse orientation

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Promoter

Cxcl1

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-R251

Plasmid

#134307

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 R251A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-R251

Plasmid

#134307

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 R251A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-S245

Plasmid

#134302

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1-S245

Plasmid

#134302

Purpose

Bacterial expression of an inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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PB-PGK-ZhcD-Cit

Plasmid

#116045

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

ZhcD-Cit

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PB-PGK-ZhcD-Cit

Plasmid

#116045

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

ZhcD-Cit

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR207-Citrine-PTS1

Plasmid

#44588

Purpose

Gateway entry vector for Citrine fluorescent protein with a peroxisome localization signal

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Tags

PTS1 (peroxisomal targeting signal type 1)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR207-Citrine-PTS1

Plasmid

#44588

Purpose

Gateway entry vector for Citrine fluorescent protein with a peroxisome localization signal

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Tags

PTS1 (peroxisomal targeting signal type 1)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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CIT gRNA (BRDN0001146852)

Plasmid

#76723

Purpose

3rd generation lentiviral gRNA plasmid targeting human CIT

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CIT (CIT Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CIT gRNA (BRDN0001146852)

Plasmid

#76723

Purpose

3rd generation lentiviral gRNA plasmid targeting human CIT

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CIT (CIT Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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