Addgene

We narrowed to 4,641 results for: LUM

Showing: 541 - 560 of 4641 results

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ER-ABKAR

Plasmid

#61508

Purpose

Expression of AMPK biosensor targeted to the endoplasmic reticulum

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

ER targeting sequence from CYP450 2C1 (see commen…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pET-mBoNT/F

Plasmid

#168015

Purpose

Expresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

MBP-BoNT/F-LC(1-428)-6xHis

Use

Tags

Hisx6 and maltose binding protein

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNAHygro(+)LAMP2AS

Plasmid

#86146

Purpose

Anti sense for LAMP2

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

LAMP2a (LAMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

contains first 525 bases of LAMP cDNA

Promoter

CMV

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-zeo_3xHA-TurboID-NLS

Plasmid

#200022

Purpose

For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

3xHA-TurboID-NLS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3Gs

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHLuc Control

Plasmid

#141091

Purpose

pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71346

Purpose

Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern(Ca2+)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pDestTol2CG2--3.5ubb:plin2-tdtomato

Plasmid

#172534

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Use

Tags

Expression

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

Plasmid

#213034

Purpose

fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Slaviero et al Neurophotonics. 2024 Apr;11(2):024208. d

Insert

mNeonGreen, ISN-IE, ChRmine

Use

AAV

Tags

Gaussia luciferase signal sequence

Expression

Mutation

Promoter

hSyn

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM7-Halo-FRB

Plasmid

#197058

Purpose

Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

Plasmid

#172535

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Use

Tags

Expression

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:AVRa1-V1

Plasmid

#132357

Purpose

Expression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

AVRa1-V1 ∆SP with stop codon

Use

Tags

Expression

Plant

Mutation

Promoter

pZmUBQ

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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P447-FBP_19

Plasmid

#139710

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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P445-FBP_17

Plasmid

#139708

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:AVRa1

Plasmid

#132356

Purpose

Expression of the Blumeria graminis f. sp. hordei effector AVRa1 ∆SP from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

AVRa1 ∆SP with stop codon

Use

Tags

Expression

Plant

Mutation

Promoter

pZmUBQ

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pcDNA3

Plasmid

#67661

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Cyan Nano-lantern/pcDNA3

Plasmid

#67660

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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35S::Cel1SP-GeNL/pCAMBIA1301

Plasmid

#190069

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Cel1 signal peptide-Green-enhanced Nano-lantern

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-CVm-PTEN

Plasmid

#168032

Purpose

Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-PTEN(M265-N329)-mVenus

Use

Tags

Hisx6 and myc

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only