We narrowed to 2,524 results for: PGK

1. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

   Plasmid

   #200475

   Purpose

   Lentiviral vector expressing gRNA targeting GFP

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP249 pGK-dCas9-DNMT3a V3

   Plasmid

   #100938

   Purpose

   dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. miniTol2 x EF1a_EKAREN5 + PGK-blast

   Plasmid

   #167818

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

   Plasmid

   #208548

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(13) (GATA3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W

   Plasmid

   #208547

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(12) (GATA3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCCLc-U6-shHMGA2.3-PGK-dTomato

   Plasmid

   #89606

   Purpose

   Silencing Hmga2 in human cells

   Depositor

   Fernando Fierro

   Article

   Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

   Insert

   shRNA targeting HMGA2 (HMGA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

   Plasmid

   #67983

   Purpose

   Cas9 activity reporter (control) with GFP and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-PGK-RAP1A-WT-neo

   Plasmid

   #156173

   Purpose

   Lentiviral vector for expression of RAP1A-WT, with neomycin section

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti PGK OGT (Neo)

   Plasmid

   #154289

   Purpose

   Lentiviral vector encoding full length wildtype human OGT

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL-GFPGO-PGK-Neo

    Plasmid

    #136890

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PITX3-2A-eGFP-PGK-Puro

    Plasmid

    #31943

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    -

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti.PGK.blast-Flag-MFF-S155,172D

    Plasmid

    #74442

    Purpose

    Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Asp, Ser 172 Asp (Numbering based on MFF …

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

    Plasmid

    #105039

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCCLc-U6-shHMGA2.2-PGK-dTomato

    Plasmid

    #89605

    Purpose

    Silencing Hmga2 in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    shRNA targeting HMGA2 (HMGA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(SOX2_v32_7-4)-PGKpuroBFP-W

    Plasmid

    #211986

    Purpose

    Express gRNA against SOX2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting SOX2 (SOX2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(SOX2_v32_7-5)-PGKpuroBFP-W

    Plasmid

    #211987

    Purpose

    Express gRNA against SOX2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting SOX2 (SOX2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-hPGK-mCherry-SynaptoZip

    Plasmid

    #177317

    Purpose

    Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF hPGK mc2 IRES neo

    Plasmid

    #206236

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EFS eGFP hPGK BSD

    Plasmid

    #188520

    Purpose

    Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge