We narrowed to 9,094 results for: Pol;

1. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

   Plasmid

   #165450

   Purpose

   Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9N-IntN
   3x Opn1mw promoter-targeting sgRNAs
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and short human rhodopsin promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CAG-CasRX-P2A-EGFP-BGH PolyA

   Plasmid

   #154004

   Purpose

   Express CasRx

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   CasRx
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

   Plasmid

   #168291

   Purpose

   optimized miniCMV (OminiCMV)

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   miniCMV

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-TgKI-MCS-p2A-tdTomato-polyA

   Plasmid

   #174027

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-tdTomato
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-CMV-EGFP-PolB-PAMmut-Hygro

   Plasmid

   #176086

   Purpose

   EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in the PAM site used by PolBKOg1

   Promoter

   CMV

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-EGFP barcode-5-SV40 polyA

   Plasmid

   #190869

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode5
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

   Plasmid

   #208964

   Purpose

   Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-TgKI-MCS-mScarlet-MCS-polyA

   Plasmid

   #174023

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   mScarlet
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. single polypeptide FPX biosensor for both caspase-3 and -8

   Plasmid

   #60886

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-3 and 8
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

10. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Flag-Polß(D256A)-IRES-puro

    Plasmid

    #128677

    Purpose

    mammalian expression of Flag-Polß(D256A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D256A

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-EGFP barcode-10-SV40 polyA

    Plasmid

    #190874

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode10
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. RNA polymerase beta prime subunit FLAG

    Plasmid

    #102337

    Purpose

    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.

    Depositor

    Erin O'Shea

    Article

    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1

    Insert

    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    

    Use

    Tags

    3x FLAG with 3X GSGS linker

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

    Plasmid

    #155194

    Purpose

    Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Truncated sequence of THBS1 (expresses Amino Acid…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. IX301: pMVP (L3-L2) myc epitope tag + polyA

    Plasmid

    #121753

    Purpose

    pMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    myc epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. IX601: pMVP (L3-L2) FLAG epitope tag + polyA

    Plasmid

    #121751

    Purpose

    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FLAG epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS NL4-3 envFS eGFP Δgag/pol

    Plasmid

    #101343

    Purpose

    Deletion from the start of gag until 229 bases before the cPPT

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta GagPol

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1-fDIO-mNeon-WPRE-PolyA

    Plasmid

    #121546

    Purpose

    Super-bright fluorescence protein for tracking and visualization

    Depositor

    Matthias Prigge

    Insert

    cre-dependent mNeon
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

    Plasmid

    #162949

    Purpose

    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIp211-P30-His-pol30(K127/164R)-3'

    Plasmid

    #99549

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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