We narrowed to 1,112 results for: cas12a

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pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

Plasmid

#115795

Purpose

Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrIIA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD8

Plasmid

#140619

Purpose

Expression of one of several LbCas12a crRNAs in Drosophila

Depositor

Michael Boutros

Publication

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6:3

Available since

Oct. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA

Plasmid

#117410

Purpose

LbCas12a-gRNA targeting TLR2.0

Depositor

Erik Sontheimer

Publication

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

LbCas12a gRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

Plasmid

#195549

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236363

Purpose

Overexpression of endAsCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

endAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, S542R, K548R

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236362

Purpose

Overexpression of HyperdAsCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, D256R, E321R, E371R

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236361

Purpose

Overexpression of dAsCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dAsCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

Plasmid

#115662

Purpose

Expresses AcrVA1 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR-Zeo

Plasmid

#158709

Purpose

For cloning and constitutive expression of crRNAs in mycobacterium

Depositor

Yi-Cheng Sun

Publication

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

crRNA

Use

CRISPR and Synthetic Biology; Mycobacteria expres…

Expression

Bacterial

Promoter

Hsp60

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

Plasmid

#115663

Purpose

Expresses AcrVA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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EC64420

Plasmid

#209517

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKEW303-AcrVA5

Plasmid

#115657

Purpose

Expresses AcrVA5 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-tagBFP-NLS-3xFlag

Plasmid

#236385

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbCas12a-EGFP-NLS-3xFlag

Plasmid

#236364

Purpose

Overexpression of dLbCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dLbCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-EGFP-NLS-3xFlag

Plasmid

#236367

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbCas12aRVRR-EGFP-NLS-3xFlag

Plasmid

#236365

Purpose

Overexpression of dLbCas12aRVRR in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dLbCas12aRVRR

Use

CRISPR and Lentiviral

Mutation

G532R, K538V, Y542R, K595R

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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