We narrowed to 4,695 results for: pcr

1. pSL0812 (pCRISPR(BbsI)_Vch)

   Plasmid

   #200898

   Purpose

   Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCRISPR (entry)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCri_L830-antiSH2-OptoMB-V416L

   Plasmid

   #159936

   Purpose

   Opto-MB bacterial expression

   Depositor

   Jose Avalos, Jared Toettcher

   Article

   Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

   Insert

   anti-SH2 MB with SS58 insert
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AsLOV2 V416L

   Promoter

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCri_L889-antiSH2-OptoMB-Triple

   Plasmid

   #159937

   Purpose

   Opto-MB bacterial expression

   Depositor

   Jose Avalos, Jared Toettcher

   Article

   Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

   Insert

   anti-SH2 MB with SS58 insert
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AsLOV2 V416I_G528A_N538E

   Promoter

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR-BluntII-TOPO-RhoC

   Plasmid

   #14099

   Depositor

   Richard Hynes

   Article

   Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

   Insert

   Human RhoC (RHOC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pCR8-Magneto2.0-p2A-mCherry

   Plasmid

   #74334

   Purpose

   For gateway cloning Magneto2.0-p2A-mCherry into destination vector

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTH1, FTL, Trpv4 Human, Rat)
   

   Use

   Gateway vector

   Tags

   Flag and p2A-mCherry

   Expression

   Mutation

   TRPV4: delta 760–871

   Promoter

   none

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-U6sgp53-mTSG-GFAPCre

   Plasmid

   #100276

   Purpose

   AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Insert

   GFAP promoter (Gfap, GFAP Mouse, Human)
   

   Use

   AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR-Blunt II-TOPO-PTPRD

   Plasmid

   #29705

   Depositor

   Todd Waldman

   Article

   Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

   Insert

   Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   May 26, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCR4-Dact1(1250-1601)

   Plasmid

   #52046

   Purpose

   To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.

   Depositor

   Ben Cheyette

   Article

   Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.

   Insert

   Dact1(1250-1601) (Dact1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCRISPR-Cas9/CD4-TK2

   Plasmid

   #104397

   Purpose

   The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

   Depositor

   Jeronimo Blanco

   Article

   Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

   Insert

   TK2 (TK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS537 tet-hCMV-GFPcre

    Plasmid

    #11960

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    GFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pCR3-Flag-AuroraB KD (K106R)

    Plasmid

    #108488

    Purpose

    expression of Flag-AuroraB kinase death (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII-TOPO GFR alpha 1

    Plasmid

    #119898

    Purpose

    Mouse GFR alpha 1 In situ hybridization probe

    Depositor

    Wenqin Luo, David Ginty

    Article

    Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

    Insert

    GDNF family receptor alpha-1 precursor (Gfra1 Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCRII-TOPO-ZFERV-ENV

    Plasmid

    #22399

    Depositor

    Lisa Steiner

    Insert

    ZFERV env gene (env Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCRII-Sema4a-ISH-2kb

    Plasmid

    #68029

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4A (Sema4a Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    a 2kb cDNA fragment for ISH probe synthesis

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRII-Plxnb2-ISH-2kb

    Plasmid

    #68036

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B2 (Plxnb2 Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRII-Sema4c-ISH-2kb

    Plasmid

    #68031

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4C (Sema4c Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRII-Topo U6-SpAi9L-U6SpDMDL

    Plasmid

    #78608

    Purpose

    Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9L-U6SpDMDL
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pCRII-Topo U6-SpAi9R-U6-SpDMDR

    Plasmid

    #78609

    Purpose

    Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9R-U6-SpDMDR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pCRII-Sema4d-ISH-2kb

    Plasmid

    #68032

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4D (Sema4d Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRII-Plxnb1-ISH-2kb

    Plasmid

    #68035

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B1 (Plxnb1 Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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