-
Plasmid#46933PurposeLuciferase Reporter for mutant STAT3DepositorInsertSTAT3(Y705F) (STAT3 Human)
UseLuciferaseTagsFLAGExpressionMutationTyrosine 705 to PhenylalaninePromoterTA LuciferaseAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Stat3 S727A pRc/CMV
Plasmid#8708DepositorInsertStat3 S727A (Stat3 Mouse)
UseTagsExpressionMammalianMutationS727A (exhibits o serine phosphorylation either c…PromoterAvailable sinceAug. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-LAMP1
Plasmid#227322PurposeDonor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)DepositorInsertLAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Venus2-Y705F/S727A-STAT3
Plasmid#123177PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
UseTagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationDouble Y705F and S727A substitutionsPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-VPR-dSpCas9[StaPL(TI)]
Plasmid#111508PurposeExpresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].DepositorInsertVPR-dSpCas9[StaPL(TI)]
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, and S122…PromoterPGKAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-5xUASG-iRFP-B3-timestamp-A
Plasmid#159925Purposetimestamp Template A inserted into the 3’ UTR of iRFP, in a second generation lentiviral backbone with the Vivid promoter, with RNA barcode CTG. AKA 149B3.DepositorInsertiRFP-Timestamp A
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-2xFlag-mCherry-Msp300KASH
Plasmid#170807PurposeExpresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesisDepositorInsert2xFLAG/mCherry-Msp300KASH
UseTags2xFLAG-mCherry and Msp300KASHExpressionInsectMutationPromoter5xUAS + hsp70 minimal promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK284 (Stag-3FLAG-Hygro)
Plasmid#72800PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Hygro
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Venus1-Y705F/S727A-STAT3
Plasmid#123176PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
UseTagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationDouble Y705F and S727A substitutionsPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK283 (Stag-3FLAG-Neo)
Plasmid#72799PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Neo
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (WT)
Plasmid#73963PurposeSTAG2 cDNA (CCDS43990) wild typeDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationwild typePromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mStayGold-T2A-mCherry
Plasmid#219834Purposeequimolar expression of mStaygold and mCherry in budding yeastDepositorInsertmStayGold-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP NoStarts
Plasmid#101342PurposeAll ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACGDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationNo methonines in GagPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-STAT3C-3xFlag
Plasmid#99264PurposeAdenoviral vector encoding Flag-tagged murine STAT3C (constitutive)DepositorInsertSignal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)
UseAdenoviralTags3x-FlagExpressionMutationA662C N664CPromoterCMVAvailable sinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 ymStayGold-T2A-mCherry
Plasmid#219843Purposeequimolar expression of ymStayGold and mCherry in budding yeastDepositorInsertymStayGold-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase gRNA resistant
Plasmid#163411PurposeExpresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deleted. gRNA-t…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-postASAP-p2a-ChrimsonR
Plasmid#178794PurposeExpresses voltage sensor targeted to dendrites and spines along with ChrimsonRDepositorInsertFingR.PSD95, ASAP2f and ChrimsonR
UseTagsmRuby2ExpressionMammalianMutationmutations in amino acids L146G, S147T N149R, S150…PromoterCAGAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag p38 alpha
Plasmid#20815DepositorInsertpGSTag Flag p38 alpha (Mapk14 Mouse)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMK285 (Stag-3FLAG-Bsr)
Plasmid#72801PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Bsr
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-STAG2 shRNA 3782
Plasmid#31979DepositorInsertSTAG2 (STAG2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only