We narrowed to 21,372 results for: ACE

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pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BF

Plasmid

#108338

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)WR

Plasmid

#108339

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BR

Plasmid

#108340

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFusX1-31

Plasmid

#63249

Purpose

Encodes RVD positions 1-3 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-pfkB

Plasmid

#102284

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

pfkB gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pFusX4-31

Plasmid

#63440

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
5mCUC1 pBluescript

Plasmid

#12065

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Expression

Bacterial

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pCAV035_mCherry_P2A_fsEGFP_GGsite

Plasmid

#219807

Purpose

Fluorescent Reporter for MUTYH-Specific Activity; Containing BsaI Recognition Sequence at Frameshifted EGFP; For OG:A Oligo Insertion at Golden Gate (GG) Site at Codon 34

Depositor

Alexis Komor

Article

Vasquez et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pME234

Plasmid

#238104

Purpose

Expresses ME-ABE ABE7.10-HK with SpCas9 (NG

Depositor

Alexis Komor

Article

Evanoff et al Nat Biotechnol. 2026 Mar 18. doi: 10.103

Insert

TadA*7.10(Y123H/N157K)-m_SpnCas9(D10A)

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

TadA modifications include Y123H, N157K; NG-nCas9…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.S29W.A95K.mRuby3

Plasmid

#244911

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mRuby3

Use

AAV

Mutation

S29W.A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95A.A119L.mIRFP670nano3

Plasmid

#244917

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mIRFP670nano3

Use

AAV

Mutation

A95A.A119L

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mIRFP670nano3

Plasmid

#244916

Purpose

iATPSnFR2.A95K variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mIRFP670nano3

Use

AAV

Mutation

A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mRuby3

Plasmid

#244912

Purpose

iATPSnFR2.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mRuby3

Use

AAV

Mutation

A95K

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Px-PhiYFP-triplex-28-M13-28-pA

Plasmid

#202047

Purpose

Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, Csy4 28nt sites, and cloning site for gRNA to activate downstream targets.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

PhiYFP

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-Triplex-28-M13-28-pA

Plasmid

#202041

Purpose

Encodes a non-coding transcript expressed from the EF1a promoter that has a 3' UTR containing a MALAT1 triplex, Csy4 binding sites ("28"), and a cloning site for a Cas9 gRNA.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

Triplex-28-M13-28 (noncoding)

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Px-PhiYFP-triplex-DR-M13-DR-pA

Plasmid

#202046

Purpose

Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, dCas12a DRs, and cloning site for crRNA to activate downstream targets.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

PhiYFP

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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