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pHAGE2-EF1aL-mCherry-W

Plasmid

#126703

Purpose

Constitutive, ubiquitous mCherry expression.

Depositor

Darrell Kotton

Article

McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

Insert

mCherry

Use

Lentiviral

Promoter

Ef1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf_LamB1

Plasmid

#181999

Purpose

CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf-LamB1

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.hSyn.GLPLight-ctr

Plasmid

#187468

Purpose

Expresses GLPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight-ctr

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA 66L

Plasmid

#19300

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q66L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen

Plasmid

#222648

Purpose

Vector for cloning gene of interest for in vitro transcription

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

ZsGreen

Use

In vitro transcription

Mutation

mutated stop codon

Promoter

T7

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAd-PV-NLS-DsRed

Plasmid

#17287

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-DsRed-NLS-CD (Pvalb Rat)

Use

Adenoviral

Tags

Myc

Expression

Mammalian

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Article

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Promoter

EF1a

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO‐puro‐2A‐mdx4cv‐GFP

Plasmid

#187053

Purpose

fluorescent reporter for adenine base editing of mdx4cv point mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-GAR1

Plasmid

#126873

Purpose

Expresses 3xFlag GAR1

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

GAR1 (GAR1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCiVSP(C363S)-SD64TF

Plasmid

#80334

Purpose

Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

Depositor

Yasushi Okamura

Article

Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

Insert

Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)

Use

Xenopus oocyte expression

Mutation

Changed Cysteine 363 to Serine

Promoter

SP6

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-p50(1-435)

Plasmid

#44747

Depositor

Gourisankar Ghosh

Article

Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

Insert

p50 (Nfkb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

AA 1-435

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAd-PV-NES-DsRed

Plasmid

#17286

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-NES (Pvalb Rat)

Use

Adenoviral

Tags

DSR and Myc

Expression

Mammalian

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
pJH645

Plasmid

#172169

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

Inserted amber codon immediately after initiator …

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Mito-Lapronic/pGW1

Plasmid

#140754

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Mito-Lapronic

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-D1994A

Plasmid

#25368

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Aspartate 1994 mutated to Alanine

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-PTEN-3'UTR-Wt

Plasmid

#21326

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

PTEN 3' UTR Wild Type (PTEN Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

Ubiquitin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AcrIIA4

Plasmid

#101043

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA4

Tags

GST

Expression

Bacterial

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pKO (Apr)

Plasmid

#110088

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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TRPML1-CFP

Plasmid

#18827

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

CFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only