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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 46,691 results for: cha

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Showing: 5601 - 5620 of 46691 results

  3. ZJOM22

    Plasmid
    #133629
    Purpose
    Integration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-EMC1-2GFP (EMC1 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133629-plasmid-map-sequence-id-265897

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  4. ZJOM70

    Plasmid
    #133658
    Purpose
    Integration of ERG6-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-ERG6-mCherry (ERG6 Budding Yeast)
    Tags
    mCherry
    Expression
    Yeast
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133658-plasmid-map-sequence-id-269552

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  6. pGS1222

    Plasmid
    #63791
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eGFP-Twin-Strep tag
    Promoter
    none
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63791-plasmid-map-sequence-id-106478

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  8. ZJOM86

    Plasmid
    #133634
    Purpose
    Integration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-ANP1-GFP (ANP1 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133634-plasmid-map-sequence-id-265885

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  9. pMM0646

    Plasmid
    #129007
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129007-plasmid-map-sequence-id-259441

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  10. ZJOM157

    Plasmid
    #133640
    Purpose
    Integration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-GFP-Pep12 (PEP12 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    133640-plasmid-map-sequence-id-272372

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  13. pMM0638

    Plasmid
    #129005
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129005-plasmid-map-sequence-id-261246

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  14. ZJOM1

    Plasmid
    #133632
    Purpose
    Integration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-GFP-SED5 (SED5 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    133632-plasmid-map-sequence-id-292100

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  16. ZJOM156

    Plasmid
    #133636
    Purpose
    Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    SEC7-2GFP (SEC7 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    133636-plasmid-map-sequence-id-272374

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  17. pYZ172

    Plasmid
    #98409
    Purpose
    Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete lys9 in genome by recombination
    downstream homologous region to delete lys9 in genome by recombination
    Use
    CRISPR and Synthetic Biology
    Available Since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98409-plasmid-map-sequence-id-198900

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  20. mOtop1_pGEMHE

    Plasmid
    #114674
    Purpose
    This construct was used to express mouse Otop1 in Xenopus laevis oocytes
    Depositor
    Emily Liman
    Article
    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.
    Insert
    Otopetrin 1 (Otop1 Mouse)
    Use
    For generating the mrna, which can highly express…
    Mutation
    SNP (rs49325632): Glycine 396 to Alanine
    Promoter
    T7
    Available Since
    July 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    114674-plasmid-map-sequence-id-226716

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Showing: 5601 - 5620 of 46691 results