We narrowed to 59,538 results for: cloning

1. pcDNA5/FRT/TO GFP SSA1

   Plasmid

   #19489

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   SSA1 (SSA1 Budding Yeast)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH41373

   Plasmid

   #50252

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (1.3 kb), 35s (c)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. GGE147

   Plasmid

   #120743

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_8UASxpr-TEF)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P1_8UASxpr-TEF
   

   Use

   Cloning/assembly plasmid

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GGE259

   Plasmid

   #120764

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 3 (P3_EYK300-5AB)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P3_EYK300-5AB
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGE129

   Plasmid

   #120755

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 2 (P2_EYK300-5AB)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P2_EYK300-5AB
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL50014

   Plasmid

   #50313

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   C terminal HBP tag (HA-PreScission-Biotin)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFH99

   Plasmid

   #128204

   Purpose

   classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GGE145

   Plasmid

   #120741

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_2UASxpr-TEF)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P1_2UASxpr-TEF
   

   Use

   Cloning/assembly plasmid

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR_P2R-P3_3'arm_apobb.1

   Plasmid

   #128681

   Purpose

   apobb.1 homology arm 3' gateway clone

   Depositor

   Steven Farber

   Article

   Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

   Insert

   apobb.1 (apobb.1 Zebrafish)
   

   Use

   Multi-step gateway cloning

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

    Plasmid

    #50896

    Purpose

    Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L717C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-Kan_PGKY_APikL3

    Plasmid

    #123652

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    PGKY_APikL3
    

    Use

    Golden gate

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-Kan_CD156_APikL3

    Plasmid

    #123651

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    CD156_APikL3
    

    Use

    Golden gate

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-Kan_BR32_APikL2

    Plasmid

    #123649

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    BR32_APikL2
    

    Use

    Golden gate

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-Kan_BR32_APikL3

    Plasmid

    #123650

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    BR32_APikL3
    

    Use

    Golden gate

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFH100

    Plasmid

    #128205

    Purpose

    improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH101

    Plasmid

    #128206

    Purpose

    sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry pBSDONR P4r-P2

    Plasmid

    #121528

    Purpose

    For making C-terminal mCherry fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. LIIc F 1-2 RNAi

    Plasmid

    #54402

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD401

    Plasmid

    #91828

    Purpose

    Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Pmyo-2::GFP
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Nucleotide 489 of the myo-2 promoter was changed …

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOGG203

    Plasmid

    #115504

    Purpose

    Clone of a level 1 unit which encodes a constitutively-expressed mCherry

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    pL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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