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MTK2_023

Plasmid

#123715

Purpose

Encodes pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK094-LM-AMA002.0

Plasmid

#171366

Purpose

Entry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJT119b

Plasmid

#50551

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pRN10

Plasmid

#84454

Purpose

shuttle plasmid for sarA P1-mKate expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mKate2

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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5UAS zfSynaptophysin:GFP

Plasmid

#74316

Purpose

A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

Depositor

Martin Meyer, Stephen Smith

Article

Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

Insert

synaptophysin

Tags

EGFP

Mutation

Q12H and deletion of F13 (please see depositor co…

Promoter

5UAS

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLG022 (dsr1)

Plasmid

#157900

Purpose

Expresses the dsr1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

dsr1 (SIR2-DUF4020)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBA562

Plasmid

#186237

Purpose

pTet-RfxCas13d crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Pinducer20-dCas9-SunTag-2A-BFP

Plasmid

#115779

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

dCas9-SunTag-2A-BFP

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDest-635

Plasmid

#159582

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT-MBI (JBEI-3085)

Plasmid

#87950

Purpose

the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

MevT-MBI

Expression

Bacterial

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pHEA

Plasmid

#168297

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Article

Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

Insert

Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)

Tags

6xHis, E-tag, and PelB signal peptide

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGL0_20 [panARS-opt ORI]

Plasmid

#198933

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

panARS-opt ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHelper_AraC/pBad-Tnpase (camR)

Plasmid

#213909

Purpose

Arabinose-inducible himar1C9 transposase expression - cam resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

AraC/pBad ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10_K139R

Plasmid

#127137

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K139R

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10_K138R

Plasmid

#127136

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K138R

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXP CMV/TO DEST (w360-1)

Plasmid

#17386

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pFPL-Ch

Plasmid

#61647

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Expression

Plant

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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