We narrowed to 10,309 results for: yeast

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pJo55

Plasmid

#159623

Purpose

An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.

Depositor

Vasiliy Taranov

Article

Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-C4A,C5A,C7A-GFP

Plasmid

#166838

Purpose

Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

C4A, C5A, C7A

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Strep II

Plasmid

#153512

Purpose

Strep II C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Strep II

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Aaw-Glucoamylase SP

Plasmid

#153497

Purpose

Aspergillus awamori Glucoamylase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Glucoamylase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Gga-Lysozym SP

Plasmid

#153505

Purpose

Chicken Lysozym Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Lysozym SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TEV-His-GFP11

Plasmid

#153510

Purpose

TEV-His-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TEV-His-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Prepro SP

Plasmid

#153491

Purpose

S. cerevisiae Prepro Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Prepro SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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A102-GAP-BoCCS

Plasmid

#123287

Purpose

Expression of truncated Glo3 (1-375) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Mutation

1-375

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-BoCCS-GRM

Plasmid

#123288

Purpose

Expression of truncated Glo3 (137-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Mutation

137-459

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-Par-BoCCS-GRM

Plasmid

#123289

Purpose

Expression of truncated Glo3 (296-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Mutation

296-459

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-BoCCS

Plasmid

#123290

Purpose

Expression of truncated Glo3 (137-375) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Mutation

137-375

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-GRM

Plasmid

#123291

Purpose

Expression of truncated Glo3 (373-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Mutation

373-459

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEDJ-22

Plasmid

#140904

Purpose

MarkerFree plasmid for integration of pADH1-PCP-Mxi1 into site X-4

Depositor

Michael Jensen

Article

Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

Insert

PCP-Mxi1

Promoter

ADH1

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(NT)

Plasmid

#138258

Purpose

Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-ysg(NT)

Plasmid

#138263

Purpose

Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAL- S. pombe TSC1 (1-431)

Plasmid

#132616

Purpose

expresses S. pombe TSC1 (1-431) in E. coli

Depositor

Wenqing Xu

Article

Sun et al Nat Commun. 2013;4:2135. doi: 10.1038/nc

Insert

TSC1 (1-431)

Expression

Bacterial

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEMY07AB-PRO

Plasmid

#127710

Purpose

Level 0 destination vector for a promoter with A - B scars. Clone in with BpiI

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Spt2-K166R-GFP

Plasmid

#115573

Purpose

Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166

Depositor

Michael Downey

Article

Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

Insert

SPT2

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Lysine 166 mutated to Arginine

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only
pRPL28 SpHIS5 (pNTI663)

Plasmid

#115433

Purpose

Expresses wild-type RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28(Q38P) SpHIS5 (pNTI664)

Plasmid

#115434

Purpose

Expresses Q38P RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

Q38P; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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