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pRVV689

Plasmid

#119016

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSO5

Plasmid

#106364

Purpose

A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr I.

Depositor

Jonathan Ewbank

Article

Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

Type

Empty backbone

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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Rab33bb cds in pGem-T Easy

Plasmid

#80524

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab33bb

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-PH(Btk)KD(Akt1)

Plasmid

#86636

Purpose

Expresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

PH(Btk)KD(Akt1)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,5G6T-GFP

Plasmid

#80912

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P5G6T and PM,5G6T

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-2

Plasmid

#72734

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-128]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-1

Plasmid

#72733

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-124]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-9

Plasmid

#72741

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSARSF53-343

Plasmid

#61759

Purpose

TvoVMAdC48 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdC48 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-143

Plasmid

#61760

Purpose

TvoVMAdC16 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdC16 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-HY176

Plasmid

#61761

Purpose

TvoVMAdC7 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAdC7 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-166

Plasmid

#61816

Purpose

PhoRadAdC14 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadAdC14 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-459

Plasmid

#61818

Purpose

PhoRadAN38 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadN38 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-455

Plasmid

#61819

Purpose

PhoRadAN13 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadN38 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSABAD14-142

Plasmid

#61825

Purpose

TvoVMAC16 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAC16 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHYBAD177

Plasmid

#61826

Purpose

TvoVMAC7 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAC16 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSABAD14-167

Plasmid

#61828

Purpose

PhoRadAC14 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadAC14 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSABAD14-467

Plasmid

#61977

Purpose

PhoRadAdN38 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadAdN38 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSABAD14-466

Plasmid

#61978

Purpose

PhoRadAdN13 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

PhoRadAdN13 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-218

Plasmid

#61755

Purpose

NpuDnaEN35 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

NpuDnaEN35 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-756

Plasmid

#61756

Purpose

NpuDnaEN11 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

NpuDnaEN11 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pNJB99

Plasmid

#51521

Purpose

Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Zif268:FokI

Use

Gateway entry vector

Tags

NLS

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCS-PesaR-Dlux

Plasmid

#47653

Purpose

PesaR-D promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-D

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added an additional esa box upstream of -35 site …

Promoter

PesaR-D

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pEXP5-NT-AlleyCat

Plasmid

#41715

Depositor

William DeGrado, Ivan Korendovych

Article

Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

F92E

Promoter

T7

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pFredB-PTTG1

Plasmid

#16585

Depositor

Bert Vogelstein

Article

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

PTTG1 (PTTG1 Human)

Expression

Mammalian

Mutation

3' targeting element

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Availability

Academic Institutions and Nonprofits only

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pET His10-Draper INT

Plasmid

#126670

Purpose

Expression of His10-Draper intracellular domain for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic domain

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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C119

Plasmid

#174354

Purpose

CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

gRNA sequence targeting human PABPC1 near stop codon region

Use

CRISPR

Availability

Academic Institutions and Nonprofits only

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C205

Plasmid

#174359

Purpose

For expressing siRNA-resistant frog ePAB in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Availability

Academic Institutions and Nonprofits only

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MultiGreen

Plasmid Kit

#1000000250

Purpose

The MultiGreen kit enables users to assemble GreenGate-compatible multigene plant transformation vectors.

Depositor

Wayne Parrott

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only