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Showing: 5621 - 5640 of 35544 results

  7. pCW-MRTFA-HA

    Plasmid
    #247436
    Purpose
    siRNA resistant HA-tagged mouse MRTF-A cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
    Depositor
    Richard Treisman
    Article
    Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:
    Inserts
    MRTF-A (Mrtfa Mouse)
    IRES2:EGFP
    Use
    Lentiviral; Dox-inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    siRNA resistant - no amino acid mutations
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247436-plasmid-map-sequence-id-491537

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  8. pCW-Rho(G14V)-HA

    Plasmid
    #247437
    Purpose
    HA-tagged human RhoA(G14V) cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
    Depositor
    Richard Treisman
    Article
    Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:
    Inserts
    RhoA(G14V) (RHOA Human)
    IRES2:EGFP
    Use
    Lentiviral; Dox-inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G14V mutation (constitutive active mutant)
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247437-plasmid-map-sequence-id-490945

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  10. pET28-His6-SUMO-Lyn-SH2-AviTag

    Plasmid
    #214207
    Purpose
    Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lyn A (LYN Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214207-plasmid-map-sequence-id-422791

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  11. pET28-His6-TEV-Grb2-SH2-mCherry-AviTag

    Plasmid
    #214231
    Purpose
    Bacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections.
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Grb2 SH2 domain (GRB2 Human)
    Tags
    6xHis, AviTag, and mCherry
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214231-plasmid-map-sequence-id-424761

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  12. pET28-His6-SUMO-Lck-SH2-AviTag

    Plasmid
    #214203
    Purpose
    Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lck kinase SH2 domain (LCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214203-plasmid-map-sequence-id-422773

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  13. pET28-His6-SUMO-Blk-SH2-AviTag

    Plasmid
    #214204
    Purpose
    Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Blk kinase SH2 domain (BLK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214204-plasmid-map-sequence-id-422774

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  14. pET28-His6-SUMO-Hck-SH2-AviTag

    Plasmid
    #214206
    Purpose
    Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    p60-HCK (HCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214206-plasmid-map-sequence-id-422790

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  19. pGEMHE-MmCENPT-RnHFD-4NS-GFP

    Plasmid
    #237432
    Purpose
    Expresses Mus musculus CENP-T with rat HFD and 4 mouse-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Rattus norvegicus HFD and 4 mouse-specific residues in HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    432 A to T; 463 T to I, 470 E to L, 485 L to Q in…
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237432-plasmid-map-sequence-id-474252

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  20. EW488 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(T2098L)

    Plasmid
    #236159
    Purpose
    Express NZF transcriptional activation domain fused to a deimmunized linker and mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Insert
    NZF-deImmunLink-FRB
    Expression
    Mammalian
    Mutation
    T2098L in FRB
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236159-plasmid-map-sequence-id-468430

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Showing: 5621 - 5640 of 35544 results