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pLib6.4-Aaeg_972

Plasmid

#176660

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Cquin_801

Plasmid

#176657

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Cquin_801

Plasmid

#176671

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Cquin_728

Plasmid

#176670

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dNrg1

Plasmid

#173696

Purpose

A knockout vector for the dog Nrg1.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEreg

Plasmid

#173697

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dTgfa

Plasmid

#173698

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dHbegf

Plasmid

#173699

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEgf

Plasmid

#173700

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-bleo-dEgf

Plasmid

#173840

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-puro-dHbegf

Plasmid

#173841

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-dEreg

Plasmid

#173843

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-puro-dEgfr

Plasmid

#173844

Purpose

A knockout vector for the dog Egfr.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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CPL2g/pDONR221

Plasmid

#172093

Purpose

Genomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Use

Gateway Cloning

Promoter

CPL2

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207-MUM2sp-PelA

Plasmid

#170722

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

Pectin lyase A

Use

Gateway donor vector / entry clone

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207-MUM2sp-RglB

Plasmid

#170721

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

Rhamnogalacturonan lyase B

Use

Gateway donor vector / entry clone

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207-MUM2sp-RglA

Plasmid

#170720

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

Rhamnogalacturonan lyase A

Use

Gateway donor vector / entry clone

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pZA44

Plasmid

#158522

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP (ZAR1 Mustard Weed)

Tags

eGFP

Expression

Plant

Mutation

L17E/D489V

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZA18

Plasmid

#158496

Purpose

In planta gene expression of Golden gate compatibleA. thaliana ZAR1 D489V. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Mutation

D489V

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA36

Plasmid

#158514

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA (ZAR1 Mustard Weed)

Tags

6xHA

Expression

Plant

Mutation

L17E, D489V

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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