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HCKan-O-ycrtYB

Plasmid

#65346

Purpose

construction of ycrtYB gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtYB

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-O-ycrtE

Plasmid

#65344

Purpose

construction of ycrtE gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtE

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-O-ycrtI

Plasmid

#65345

Purpose

construction of ycrtI gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtI

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSL14 - Prp8 2142–2398

Plasmid

#90264

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

PRP8 (PRP8 Budding Yeast)

Tags

N-terminal 6xHis tag

Expression

Bacterial

Mutation

only Jab1 domain (aa 2143-2398)

Promoter

T7

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD8

Plasmid

#112820

Purpose

pSMF2 with 8 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD10

Plasmid

#112821

Purpose

pSMF2 with 10 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFP140

Plasmid

#21672

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP120

Plasmid

#21673

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, a 918…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pKF251

Plasmid

#52532

Purpose

Double-GFP reporter for DNA cleavage

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Double-GFP

Expression

Insect

Promoter

ubiquitin

Available Since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

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pJT3

Plasmid

#98304

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TRPL3AcNES1>TRPL41B

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pNB775

Plasmid

#60115

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB776

Plasmid

#60117

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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Prp2-pRSETA

Plasmid

#61042

Purpose

Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Prp2

Tags

Histidine Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
YCplac111-KOG1 (pAN58)

Plasmid

#39209

Depositor

Michael Hall

Article

Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

Insert

KOG1 (KOG1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

L1512P

Promoter

KOG1

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pFP118

Plasmid

#21671

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pRY1001

Plasmid

#21787

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

CFP-Ste12 (STE12 A. victoria, Budding Yeast)

Tags

CFP

Expression

Yeast

Mutation

contains 1000 bp UTR (Ste12 promoter) upstream of…

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pYO286

Plasmid

#235730

Purpose

Protein expression of yeast VPS15 (1-981)-ZZ in yeast

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

aa 1-981

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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SP_H3

Plasmid

#228189

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P47001_CIS3_YEAST:0-21

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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SP_F2

Plasmid

#228176

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast EXG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P23776_EXG1_YEAST:0-19

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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