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pTS1107_Tier3(SB)

Plasmid

#169634

Purpose

Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase (5'ITR-A1-pA::A2-pA::A3-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRS415-recR

Plasmid

#49457

Purpose

single copy recombinase yeast expression plasmid

Depositor

Marc Gartenberg

Article

Ansari et al Methods. 1999 Feb;17(2):104-11.

Insert

recR

Expression

Yeast

Promoter

Gal1

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pUC18PV-mini-Tn7T

Plasmid

#64956

Purpose

pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC.B2.MJ0936

Plasmid

#11538

Depositor

Sung-Hou Kim

Article

Chen et al J Biol Chem. 2004 Jul 23. 279(30):31854-

Insert

phosphodiesterase

Tags

HisTag, non-cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pJKW0826

Plasmid

#119027

Purpose

E.coli expression plasmid for PmKOMT2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmKOMT2

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRAV12

Plasmid

#67713

Purpose

Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Kieft et al RNA. 2004 Jun;10(6):988-95.

Insert

Transcription cassette for 3'-tagged RNAs

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-SH2

Plasmid

#209704

Purpose

Mammalian expression of SH2

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

SH2

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL001

Plasmid

#117825

Purpose

Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_YlGUT1_YlCyc1TT

Expression

Bacterial and Yeast

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXP618

Plasmid

#26848

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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PMT-mApple

Plasmid

#203771

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

PMT-mApple

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pUC-mCA1-emptyMCS

Plasmid

#35095

Depositor

James Fleet

Article

Xue et al Mol Cancer Res. 2010 Aug;8(8):1095-104.

Type

Empty backbone

Use

Mouse Targeting

Promoter

mouse carbonic anhydrase 1

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pXP412

Plasmid

#26840

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK mazei

Plasmid

#108947

Purpose

heterologous expression of MVK of M. mazei in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBTM116-D9

Plasmid

#111232

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Article

Worseck et al Methods Mol Biol. 2012;812:63-87. doi: 1

Insert

ccdB-cassette

Expression

Yeast

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1131

Plasmid

#119028

Purpose

E.coli expression plasmid for PmKLR1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmKLR1

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pXP518

Plasmid

#26846

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pBS Coupler 1

Plasmid

#185824

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET-G2K

Plasmid

#70169

Purpose

bacterial expression of G2K (d-glycerate 2-kinase)

Depositor

George Reed, Paul Sims

Article

et al Journal of Molecular Catalysis B: Enzyma

Insert

garK/G2K

Tags

6xHIS and V5

Expression

Bacterial

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PM mir shLEF1 b

Plasmid

#27018

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 1b

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only