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pMJ123: pRS426-His-MBP-SpuFz1_PSP1

Plasmid

#205256

Purpose

S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pIM573

Plasmid

#74637

Purpose

alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Expression

Yeast

Promoter

none

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc

Plasmid

#108234

Purpose

Broad host bacterial expression vector with constitutive Pc promoter.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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ROPY (sYFP2-OP18-mScI-R125I)

Plasmid

#128002

Purpose

Improved redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin

Depositor

Dorus Gadella

Article

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

sYFP2-OP18-mScI-R125I

Expression

Mammalian

Mutation

R125I in mScarlet-I

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_60/pcDNA3

Plasmid

#85207

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_60

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRLuc110UGA

Plasmid

#41729

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

RLuc110UGA

Use

Luciferase

Expression

Mammalian

Mutation

in-frame UGA stop codon at codon 110 in the open …

Promoter

CMV

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pBAD/His-miRFP709

Plasmid

#79986

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLPC Flag-mRAP1

Plasmid

#73135

Purpose

Expression of Flag-tagged mRAP1

Depositor

Titia de Lange, Agnel Sfeir

Article

Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

Insert

Rap1 (Terf2ip Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMJ332: pU6-GtFz1_omegaRNA-entry

Plasmid

#205265

Purpose

Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG308-His-redFAST

Plasmid

#160350

Purpose

Expresses His-tagged redFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-redFAST

Expression

Bacterial

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pN1-QNA

Plasmid

#129309

Purpose

Negative control for ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-NA

Expression

Mammalian

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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UPG

Plasmid

#161003

Purpose

ubiquitous expression of PCP-GFP

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PCP-GFP

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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OeNL_pRSETb

Plasmid

#89529

Purpose

Orange color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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JAB2240

Plasmid

#190359

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::PhlF-NLS-19Sterm)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::PhlF-NLS-19Sterm

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pIM23

Plasmid

#74643

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

MET25

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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mTFP1-Lifeact-7

Plasmid

#54749

Purpose

Localization: Actin, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Lifeact-mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
px330 EZH2 - pDY052

Plasmid

#171107

Purpose

Expresses Cas9 and an sgRNA targeting EZH2

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Th

Plasmid

#105988

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

tyrosine hydroxylase (Th Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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miniCMV-iRFP670-tDeg

Plasmid

#185403

Purpose

iRFP670-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

iRFP670-tDeg

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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