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pYPQ131A2.0

Plasmid

#99884

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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Salsa-BC1-P1A1

Plasmid

#202842

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC1

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-lacZ-stop-L2

Plasmid

#186359

Purpose

Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ Synthetic, E. coli)

Use

Expression of lacz reporter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA E-cadherin

Plasmid

#18802

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin shRNA (CDH1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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pTnsABC

Plasmid

#160031

Purpose

Expresses V. cholerae TnsABC

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Mutation

no

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115268

Purpose

Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

EiCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKI-GFP

Plasmid

#118482

Purpose

GFP-tagged Protein Kinase A inhibitor peptide.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

PKI-GFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLAMP1-miRFP670nano

Plasmid

#127435

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

LAMP1-miRFP670nano

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAC026

Plasmid

#227598

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

Depositor

Brittany Adamson

Article

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1b

Plasmid

#80612

Purpose

pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx1(yeast)

Plasmid

#65004

Purpose

Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1

Tags

roGFP2

Expression

Yeast

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

Plasmid

#175547

Purpose

Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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CX-nestinGFPas

Plasmid

#32401

Depositor

Patrick Salmon, Henryk Zulewski

Article

Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

Insert

nestin promoter/enhancer GFP

Use

Lentiviral

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP703

Plasmid

#79993

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Lifeact

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pEE391

Plasmid

#149281

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_FT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221-Renilla

Plasmid

#79496

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Renilla Luciferase

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ132A2.0

Plasmid

#99887

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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