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pRS416 Gal-RNQ1 153-405-YFP

Plasmid

#18689

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 153-405

Tags

YFP

Expression

Yeast

Mutation

Prion domain of Rnq1

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-Brd4 R1337P

Plasmid

#21939

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, R1337P

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pFasBac+GFP-CAMSAP2 CC+CKK

Plasmid

#59039

Purpose

for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CC+CKK domains (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pRRG43-sFRP1

Plasmid

#99071

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea sFRP1 (secreted frizzled related protein 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

sFRP1 (secreted frizzled related protein 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcihygrosynDAT

Plasmid

#19992

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

synDAT (SLC6A3 Human)

Expression

Mammalian

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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hrGFP-BATS

Plasmid

#30496

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Barkor (ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

413-492 aa

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC+CKK

Plasmid

#59048

Purpose

for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 534–1457

Plasmid

#59050

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pDisplay FLIPE-100u

Plasmid

#13547

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-100u

Plasmid

#13539

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Bacterial

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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rGSKmyc-pXT7

Plasmid

#15492

Depositor

Sergei Sokol

Article

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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nPEX-LV

Plasmid

#12119

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Mutation

noncatalytic fragment of MMP2 amplified via PCR, …

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
psicheck-2-Sfxn3

Plasmid

#48169

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of SFXN3 (SFXN3 Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-Brd4 S1351R

Plasmid

#21941

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa1209-1362 only, S1351R

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
Barkor Δ10

Plasmid

#30500

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Barkor (ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

1-483aa

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
pJM601-TRA-2::FLAG

Plasmid

#27129

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 FLAG (tra-2 Nematode)

Tags

3x FLAG

Expression

Worm

Mutation

Replaced TRA-2 stop codon with KpnI and EcoRI and…

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
SMAD4 KO Neo

Plasmid

#16646

Depositor

Bert Vogelstein

Article

Zawel et al Proc Natl Acad Sci U S A. 2002 Mar 5. 99

Insert

Smad4 knock-out construct (SMAD4 Human)

Expression

Bacterial

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
GST eIF2beta

Plasmid

#45900

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 (Eif2s2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-Brd4 D1346N

Plasmid

#21940

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, D1346N

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only