We narrowed to 17,417 results for: mal.2

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Rtn2b-NTD1-271-mNeon

Plasmid

#186601

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 271 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-135-mNeon

Plasmid

#186602

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 135 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 135 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-60-mNeon

Plasmid

#186603

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 60 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 60 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP

Plasmid

#169588

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin-2

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-ABE8e

Plasmid

#185671

Purpose

Expresses SpRY-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY

Use

CRISPR

Expression

Mammalian

Mutation

SpRY D10A

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Lyn11-targeted FRB (LDR)

Plasmid

#20147

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-linker-FRB (LDR) (MTOR Human)

Expression

Mammalian

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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Stat1 alpha Y701F Flag pRc/CMV

Plasmid

#8702

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha Y701F (STAT1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Y701F

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
Stat1 alpha S727A pRc/CMV

Plasmid

#8700

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha S727A (STAT1 Human)

Expression

Mammalian

Mutation

S727A

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
Stat1 alpha Y701F pRc/CMV

Plasmid

#8701

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha Y701F (STAT1 Human)

Expression

Mammalian

Mutation

Y701F

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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px330-MRPsg2

Plasmid

#87190

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 2

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #2 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJAF216

Plasmid

#52405

Purpose

Expresses Flag-tagged mouse Arf4

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf4 (Arf4 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pJAF210

Plasmid

#52406

Purpose

Expresses Flag-tagged mouse Arf5

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf5 (Arf5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pLV-Rtn2b-R60f-mCherry

Plasmid

#186607

Purpose

Third generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD136-271-mNeon

Plasmid

#186604

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

Plasmid

#128758

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #3 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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LX-miR

Pooled Library

#112200

Purpose

Targets 1,594 primary human miRNA genes. The library contains 4-5 gRNAs per gene and 1,000 non-targeting control gRNAs for a total of 8,382 gRNAs.

Depositor

Ren-Jang Lin

Article

Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/Strep-HA-ZAK beta

Plasmid

#141195

Purpose

Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 isoform 2 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pYeDP60-hATP13A2-E343A

Plasmid

#171378

Purpose

A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

Depositor

Peter Vangheluwe

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

human ATP13A2 variant 2 wild-type

Tags

BAD tag

Expression

Yeast

Mutation

E343A

Promoter

URA3

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD300E-Fc(DAPA)-AviTag-6xHis

Plasmid

#156549

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD300E (CD300E Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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