We narrowed to 17,427 results for: EMB

1. qTAG-C-mStayGold-Blast

   Plasmid

   #227254

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNO286-3

   Plasmid

   #107746

   Purpose

   Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

   Depositor

   Jeffrey Tabor

   Article

   Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

   Inserts

   CcaS
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Deleted AA 223 and 512

   Promoter

   J23106 and J23108

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOlyA(WT)

   Plasmid

   #132391

   Purpose

   Ostreolysin A for sensing sphingomyelin/cholesterol complexes

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A wild-type
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, C94S, S151C

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Paxillin-pEGFP

   Plasmid

   #15233

   Depositor

   Rick Horwitz

   Article

   Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

   Insert

   paxillin (PXN Chicken)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Puro-mScarlet

   Plasmid

   #207689

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. qTAG-C-mStayGold-Puro

   Plasmid

   #227253

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCI-T7Max-UTR1-deGFP-8xHis-T500

   Plasmid

   #178422

   Purpose

   Expression of deGFP via a T7 Promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

   Insert

   deGFP
   

   Use

   Synthetic Biology

   Tags

   8xHis Tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. iRFP670-PIP4K2C

   Plasmid

   #202750

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   iRFP670:PIP4K2C (PIP4K2C Human)
   

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PTEN-C124S

   Plasmid

   #28300

   Depositor

   Maria-Magdalena Georgescu

   Article

   Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

   Insert

   PTEN (PTEN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Phosphatase Inactive Mutant Cys124Ser

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pRU41

    Plasmid

    #167663

    Purpose

    ENTRY vector for gRNA1 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRU42

    Plasmid

    #167664

    Purpose

    ENTRY vector for gRNA2 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LV-GFP

    Plasmid

    #25999

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-GFP (H2BC21 A. victoria, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRU43

    Plasmid

    #167665

    Purpose

    ENTRY vector for gRNA3 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-N-Puro-mStayGold

    Plasmid

    #227250

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19-T7-3WJdB-T

    Plasmid

    #87308

    Purpose

    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Three-way junction dimeric Broccoli aptamer
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-C-mStayGold-Zeo

    Plasmid

    #227255

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. qTAG-C-3xFLAG-Puro

    Plasmid

    #207728

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. qTAG-C-mScarlet-Puro

    Plasmid

    #207722

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. mEGFP-PLIN3

    Plasmid

    #222426

    Purpose

    To express PLIN3 with mEGFP tag in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    PLIN3 (PLIN3 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-V5-Puro

    Plasmid

    #207730

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge