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pcDNA5 FRT/TO Plk4-mCherry

Plasmid

#80269

Purpose

mammalian expression plasmid for Plk4-mCherry

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-GFP

Plasmid

#112851

Purpose

General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-GFP

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Blast

Plasmid

#207716

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCMB-ACTFr

Plasmid

#61192

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-Blast

Plasmid

#207711

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMLRT

Plasmid

#78234

Purpose

Expresses Murine Leukemia Reverse Transcriptase (MLRT)

Depositor

Christopher Voigt

Article

Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

Insert

Murine leukemia reverse transcriptase

Expression

Bacterial

Promoter

J23102

Available Since

Aug. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMK231 (AAVS1 CMV-MCS-PURO)

Plasmid

#105924

Purpose

Donor for integration at the human AAVS1 locus

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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hMTP-flag

Plasmid

#138335

Purpose

Expresses MTP

Depositor

Mahmood Hussain

Article

Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

Insert

MTTP (MTTP Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2

Plasmid

#18047

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mScarlet

Plasmid

#207689

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU47

Plasmid

#167669

Purpose

ENTRY vector for gRNA7 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-Puro

Plasmid

#207722

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTn7-SCOUT11.R

Plasmid

#201003

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

mCherry

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEF6a-CD28-PafA

Plasmid

#113400

Purpose

Expresses CD28-PafA in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

CD28-PafA

Tags

myc

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBTSG

Plasmid

#159420

Purpose

Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mRFP-Clc

Plasmid

#14435

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Clathrin light chain (Clc Mouse)

Tags

mRFP

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pFLAG ARMS

Plasmid

#24086

Depositor

Moses Chao

Article

Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

Insert

ARMS (Kidins220 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-ultraID

Plasmid

#207682

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEAK10-His-Myc-Kal12

Plasmid

#25442

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal12

Tags

His and Myc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only