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Venus-155-239-Gbeta1

Plasmid

#234751

Purpose

Mammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)

Depositor

Nevin Lambert

Article

Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

Insert

GNB1 (GNB1 Human)

Tags

VN155

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-mCherry

Plasmid

#127538

Purpose

Expresses human SOX2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMRXIB-mRuby3-STX17TM

Plasmid

#221744

Purpose

Stable expression of mRuby3-STX17TM in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

STX17 (STX17 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

deleted amino acids 1-228

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt-Electra2-P2A-mScarlet

Plasmid

#196114

Purpose

zebrafish expression of nbt-Electra2-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-Electra2-P2A-mScarlet

Use

Zebrafish expression

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xFLAG-2xSTREP TFDP2

Plasmid

#236445

Purpose

transient overexpression of TFDP2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

TFDP2 (TFDP2 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pPaxillin-Electra1-N1

Plasmid

#179484

Purpose

Expresses paxillin fused to blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

paxillin-Electra1

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-140

Plasmid

#118524

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-147

Plasmid

#118563

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEC3079 (p7INT-Pveg_lacI)

Plasmid

#218505

Purpose

integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

lacI (lacI Escherichia coli)

Expression

Bacterial

Promoter

Pveg

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z29

Plasmid

#120072

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-152

Plasmid

#118534

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAsCpf1(TYCV)(BB) (pY211)

Plasmid

#89352

Purpose

Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CBh

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1

Plasmid

#17743

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1 (PIK3R1 Human)

Expression

Insect

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pSF-p15A-YFP

Plasmid

#186563

Purpose

p15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Tags

None

Expression

Bacterial and Mammalian

Mutation

None

Promoter

Promotorless

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet2

Plasmid

#85743

Purpose

shRNA against mouse Tet2

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet2

Use

AAV

Tags

EYFP

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCytERM-Electra2

Plasmid

#179477

Purpose

ER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10D(E215Q)-HA

Plasmid

#209248

Purpose

Mammalian expression of ATP10D mutant E215Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10D (ATP10D Human)

Tags

HA

Expression

Mammalian

Mutation

E215Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-Sec24D

Plasmid

#66614

Purpose

YFP fusion of Sec24D

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24D (SEC24D Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pMRE-Tn5-134

Plasmid

#118520

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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