We narrowed to 9,277 results for: Pol;

1. pBactin-mOrange2-CI

   Plasmid

   #196852

   Purpose

   Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

   Plasmid

   #197969

   Purpose

   Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   L1332V, delta(1616-1666)

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

   Plasmid

   #197970

   Purpose

   Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   C328S, C331S, L1332V, delta(1616-1666)

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. DsRed -7e

   Plasmid

   #191765

   Purpose

   Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Tags

   N terminal fusion of negatively charged polypetod…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. H1.M10.luc

   Plasmid

   #193706

   Purpose

   Expressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   H1-core promoter with multiple 7SK domains (PSE, spacer, and TATA box) + N41
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV.CBA.YFP.miR-E_shSrpk3-1

   Plasmid

   #180400

   Purpose

   Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shSrpk3-1 (Srpk3 Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CBA

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV.CBA.YFP.miR-E_shSrpk3-2

   Plasmid

   #180401

   Purpose

   Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shSrpk3-2 (Srpk3 Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CBA

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV.CBA.YFP.miR-E_shSrpk3-3

   Plasmid

   #180402

   Purpose

   Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shSrpk3-3 (Srpk3 Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CBA

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV.CBA.YFP.miR-E_shStk16-2

   Plasmid

   #180392

   Purpose

   Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shStk16-2 (Stk16 Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CBA

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV.CBA.YFP.miR-E_shStk16-3

    Plasmid

    #180393

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-3 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL5.0-EGFP-HRasC20

    Plasmid

    #187252

    Purpose

    Lenticiral expression of EGFP-HRasC20

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Elife. 2021 Oct 18;10. pii: 67915. doi:

    Insert

    HRasC20 (HRAS Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    5' LTR

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST_YlHD (pBS0275)

    Plasmid

    #185153

    Purpose

    For the mammalian expression of the Yarrowia fungus protein YlHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    YlHD
    

    Expression

    Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-2T-CBP(1-287)

    Plasmid

    #182839

    Purpose

    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    CBP1-287
    

    Expression

    Bacterial

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CDS12_Cas9-NLS

    Plasmid

    #136122

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_AtcoCas9-NLS(noStop)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-bNup98-DN-NLS-mClover3

    Plasmid

    #171495

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Nup98-DN (NUP98 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV BARK R106A p2A mCherry

    Plasmid

    #117688

    Purpose

    CMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs R106A peptide
    

    Tags

    HA / FLAG

    Expression

    Mammalian

    Mutation

    R106A

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CPH3566 (pDEST15_Spt6 1223-1451)

    Plasmid

    #91814

    Purpose

    Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 1-1222

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CPH1141 (pET151_Spt6 1247-1451)

    Plasmid

    #91815

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    deleted residues 1-1246

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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