We narrowed to 17,000 results for: puromycin

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pLenti-CMV-puro_FlnaABD-TS-16flag

Plasmid

#234868

Purpose

Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

Depositor

Michael Murrell

Article

Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

Insert

FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16

Use

Lentiviral

Tags

GFP, RFP, FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

Plasmid

#223175

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-EGFP-ADRP

Plasmid

#87157

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

mCherry-R TTGGTCACCTTCAGCTTGG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_Gateway-3xFLAG

Plasmid

#172981

Purpose

Gateway Destination vector for doxycycline-inducible expression of ORF

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-puro/HA-FLAG-RBP2

Plasmid

#14802

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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Lenti-multi-CRISPR

Plasmid

#85402

Purpose

Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pJL240

Plasmid

#243695

Purpose

A piggyBac vector containing the COX5B genomic sequence, modified with silent mutations to allow for PCR-based analysis

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

COX5B (COX5B Human)

Expression

Mammalian

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJL306

Plasmid

#243703

Purpose

A piggyBac vector containing the NDUFB9 coding sequence (CDS) flanked by the 5' and 3' UTRs of the Actb gene

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

ACTB 5'UTR-NDUFB9 CDS-ACTB 3'UTR (NDUFB9 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shRNA E-cadherin

Plasmid

#18801

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin shRNA (CDH1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

Plasmid

#125256

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

Depositor

Roger D. Kamm

Article

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2(H134R)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF1a-forward

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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HA-VHL wt-pBabe-puro

Plasmid

#19234

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-U2AF2-TurboID-HA-rtTA

Plasmid

#226997

Purpose

Integrative plasmid to express U2AF2-TurboID in mammalian cells

Depositor

Bruno Dallagiovanna

Article

Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

Insert

U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)

Tags

TurboID-HA

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

Plasmid

#31483

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro

Use

Lentiviral

Mutation

Traffic Light 2.0 series have a +3 PEST sequence …

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-MYH9

Plasmid

#168273

Purpose

Expresses MYH9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

Plasmid

#68375

Purpose

Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

human PGK1

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 WT-Puro

Plasmid

#123319

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pPBpuro-EGFR(M721)

Plasmid

#197379

Purpose

Encoding human ErbB1 mutant.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR mutant (EGFR Human)

Expression

Mammalian

Mutation

Lysine 721 to Methionine

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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