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AT2G33170 X049_pECIA2

Plasmid

#115101

Purpose

Bait vector AT2G33170 X049_pECIA2 should be used with prey vector AT2G33170 X049_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G33170 (AT2G33170 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G74360 X038_pECIA2

Plasmid

#115080

Purpose

Bait vector AT1G74360 X038_pECIA2 should be used with prey vector AT1G74360 X038_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G74360 (AT1G74360 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT5G63930 X059_pECIA14

Plasmid

#114916

Purpose

Prey vector AT5G63930 X059_pECIA14 should be used with bait vector AT5G63930 X059_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G63930 (AT5G63930 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G17230 X074_pECIA14

Plasmid

#114896

Purpose

Prey vector AT1G17230 X074_pECIA14 should be used with bait vector AT1G17230 X074_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G17230 (AT1G17230 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G33170 X049_pECIA14

Plasmid

#114901

Purpose

Prey vector AT2G33170 X049_pECIA14 should be used with bait vector AT2G33170 X049_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G33170 (AT2G33170 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G74360 X038_pECIA14

Plasmid

#114880

Purpose

Prey vector AT1G74360 X038_pECIA14 should be used with bait vector AT1G74360 X038_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G74360 (AT1G74360 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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CIRTS-Gas5

Plasmid

#213172

Purpose

CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.

Depositor

Timothy Bredy

Article

Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

Insert

CIRTS-Calm3-U6-Gas5 gRNA

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

Syn1, U6

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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JpExpress404 PTEN

Plasmid

#49420

Purpose

Bacterial Expression Vector

Depositor

Ramon Parsons

Article

Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

Insert

PTEN (PTEN )

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pENTR1A-HA-Jag1

Plasmid

#46052

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Gateway Cloning

Tags

HA tag

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
MSCVhyg-HA-FLAG_Ubc9_WT

Plasmid

#164936

Purpose

Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK2 R67S

Plasmid

#53172

Purpose

Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Abl-SH2

Plasmid

#46402

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Abl (ABL1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 120-225

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pX330-Elf5 gRNA

Plasmid

#128836

Purpose

gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

mElf5 gRNA (Elf5 Mouse)

Use

CRISPR

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL2 CTF-FLAG

Plasmid

#217707

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRL2 (ADGRL2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-824

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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AT4G23740 J11_pECIA2

Plasmid

#114998

Purpose

Bait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G23740 (AT4G23740 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLX311 HRAS

Plasmid

#117749

Purpose

Open reading frame vector encoding HRAS

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

RASH1 (HRAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Closed vector so will not read through the V5 reg…

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK2 D321N

Plasmid

#53173

Purpose

Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Gata3

Plasmid

#128824

Purpose

dox-dependent expression of mouse Gata3

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Gata3 (Gata3 Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.ARID3a.IRES.GFP

Plasmid

#169300

Purpose

Constitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

ARID3A (ARID3A Human)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

SFFV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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PB-PE xCas9

Plasmid

#172652

Purpose

All-in-one prime editor piggyBac transposon (xCas9)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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