We narrowed to 7,705 results for: tev

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pNTI194

Plasmid

#84266

Purpose

Expresses Avi-tagged RPL1 and humanized birA

Depositor

Nicholas Ingolia

Article

Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

Inserts

RPL1 (RPL10A (*see below), Human)
humanized birA

Tags

Avi tag, Flag, Gly/Ser linker, and TEV site

Expression

Mammalian

Mutation

R33A, A34E (*see below)

Promoter

CMV and P2A peptide

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCAD29_hIRG1_4-461_pvp008

Plasmid

#124843

Purpose

Expresses human CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 462-end.

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC4 CD

Plasmid

#224251

Purpose

Expresses human KDAC4 (HDAC4) catalytic domain in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC4 (HDAC4 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 648-1083 (catalytic domain…

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC7 CD

Plasmid

#224341

Purpose

Expresses human KDAC7 (HDAC7) catalytic domain in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC7 (HDAC7 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 521-942 (catalytic domain).

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSDC803_IKZF1_mutant

Plasmid

#224113

Purpose

Insect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blotting

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

IKZF1 (IKZF1 Human)

Tags

Flag-TEV

Expression

Insect

Mutation

Residues 140-196, Q146A and G151N

Promoter

polyhedrin

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-SH3(2)-5R

Plasmid

#112089

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-5R (NCK1 Human)

Tags

His6 and MBP

Expression

Bacterial

Mutation

construct contains only repeats of the second SH3…

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-HT-LC3-SV40-Hygro

Plasmid

#182045

Purpose

Lentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closure

Depositor

Hong-Gang Wang

Article

Takahashi et al J Cell Biol. 2019 Oct 7;218(10):3336-335

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

HaloTag-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAD39_mIRG1_4-462_pvp008

Plasmid

#124873

Purpose

Expresses mouse CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (Acod1 Mouse)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 463-end.

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac-GFP-dynein-2(D1091–Q4307)

Plasmid

#64064

Purpose

Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

Depositor

Andrew Carter

Article

Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

Insert

Cytoplasmic dynein-2 (DYNC2H1 Human)

Tags

GFP, His tag, and ZZ tag

Expression

Insect

Mutation

Deleted amino acids 1-1090, extra C-terminal vali…

Promoter

Polyhedrin

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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His-MBP human SPOP (aa 28-337)

Plasmid

#52294

Purpose

bacterial expression of human SPOP (aa 28-337) fused to His-MBP

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

SPOP (SPOP Human)

Tags

His-MBP and TEV

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
ASC-CASP1 Octamer

Plasmid

#164032

Purpose

Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CASP1, ASC (CASP1, PYCARD Human)

Tags

His6-MBP-TEV

Expression

Bacterial

Mutation

W169G (ASC), G20K (CASP1)

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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SYKA-c020

Plasmid

#175489

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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LIC1B_Caspase-LOVN7C-7 C450A

Plasmid

#104628

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Tags

His and LOV2

Expression

Bacterial

Mutation

C450A (dark-locked and light-insensitive mutation)

Promoter

T7

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac (GST-SNAP-Shp2)

Plasmid

#177897

Purpose

Baculo expression of GST tag fused to SNAP tag and human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Tags

TEV-GST-PreScission cut site-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC8 H143A

Plasmid

#224344

Purpose

Expresses human KDAC8 (HDAC8) H143A in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 143 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJJGL004

Plasmid

#180608

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

PlmCasX with DpbCasX R1 loop

Use

CRISPR

Tags

10xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST

Plasmid

#182547

Purpose

Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificity

Depositor

Robert de Vries

Article

Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

Insert

H5_Vietnam_1203_04_Y161A

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Y161A mutant that confers exclusive NeuAc to NeuG…

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Shp2)

Plasmid

#177899

Purpose

Baculo expression of His10 tag fused to human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Tags

10xHis-TEV-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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