We narrowed to 8,706 results for: GAL

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pLX-B2 (GB3374)

Plasmid

#160636

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRbeta

Plasmid

#136455

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRB

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRbeta (PDGFRB Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-LexGAD-3xP3-RFP

Plasmid

#127558

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

LexGAD

Use

CRISPR

Expression

Insect

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pafG-CBE-3T

Plasmid

#220374

Purpose

for hA3A-nCas9 and gRNA expression in Aspergillus flavus

Depositor

Xue Gao

Article

Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42

Inserts

hA3A-nCas9
tRNA and gRNA scaffold

Use

CRISPR

Promoter

Ptef1 and tRNA

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Plasmid

#115684

Purpose

NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

Depositor

David Suter

Article

Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

Insert

Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Use

Lentiviral

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CD2APtk

Plasmid

#86004

Purpose

homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

DsRedEx_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-B3omega1 (GB1694)

Plasmid

#160643

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p(A)n-PAUSE

Plasmid

#118068

Purpose

Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

Plasmid

#229787

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; ZF10-NS3-p65

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRedF

Plasmid

#118057

Purpose

GBPart - RedF Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Red Firefly Luciferase CDS

Use

Synthetic Biology; Domestication of dna parts for…

Mutation

Red mutant S286Y

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJR031

Plasmid

#198825

Purpose

Stable step function ChR2 variant. Activated by bluelight, terminated by green light.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd (noNLS) nog

Plasmid

#119554

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_Down-Tandem Donor

Plasmid

#229783

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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HPF1-c004

Plasmid

#216874

Purpose

Bacterial expression vector for E. coli producing full length HPF1 protein with N-terminal YFP fusion

Depositor

Lari Lehtiö

Article

Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

Insert

Full length HPF1 (HPF1 Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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p35S:Citrine-TurboID-3xHA

Plasmid

#199243

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID

Tags

3xHA and TurboID-3xHA

Expression

Plant

Promoter

CaMV 35S

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJL049

Plasmid

#198809

Purpose

In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR

Expression

Worm

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem

Plasmid

#229794

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Adeno EA

Plasmid

#64071

Purpose

Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Insert

U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9

Use

Adenoviral

Tags

FLAG-Cas9

Promoter

U6 and CBh

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pJR017

Plasmid

#198835

Purpose

In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::TeTX-SL2-mKate::let-858 3'UTR

Expression

Worm

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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