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MAD2L1BP F1.1 gRNA

Plasmid

#90744

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F1.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F2.1 gRNA

Plasmid

#90745

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt-AH(R25C)

Plasmid

#39534

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (PH domain only) (Akt1 Mouse)

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

PH domain only with R25C mutation

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
Stat92e pBluescript SK(+)

Plasmid

#8712

Depositor

Jim Darnell

Article

Yan et al Cell 1996 Feb 9;84(3):421-30.

Insert

Stat92e (Stat92E Fly)

Expression

Bacterial

Available Since

July 8, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-Renilla-FLAG-firefly

Plasmid

#255006

Purpose

Used as a control for Plasmid pcDNA3 Renilla-FLAG-firefly-K529E

Depositor

Joshua Mendell

Insert

Renilla-FLAG-firefly

Use

Luciferase

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-PCNA

Plasmid

#199702

Purpose

Visualization of cell cycle progression and subdivision of S phase living mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Mol Biol 2016, 5:2. doi: 10.4172/2168-95

Insert

PCNA (PCNA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pJM118-CFP-LacI-AR_NTD_Human

Plasmid

#249248

Purpose

For overexpression of CFP-LacI-AR-NTD

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-AR-NTD

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pJM118_CFP-hFUS-IDR

Plasmid

#249247

Purpose

For overexpression of CFP-LacI-hFUD-IDR

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-hFUD-IDR

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pJM118-CFP-LacI-NR3C2(MR)_NTD_Human

Plasmid

#249251

Purpose

For overexpression of CFP-LacI-MR-NTD

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-MR-NTD

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pJM118-CFP-LacI-ER_NTD_Human

Plasmid

#249249

Purpose

For overexpression of CFP-LacI-ER-NTD

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-ER-NTD

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pJM118-CFP-LacI-PGR(PR)_NTD_Human

Plasmid

#249252

Purpose

For overexpression of CFP-LacI-PR-NTD

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-PR-NTD

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pJM118-CFP-LacI-NR3C1(GR)_NTD_Human

Plasmid

#249250

Purpose

For overexpression of CFP-LacI-GR-NTD

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-GR-NTD

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B WT

Plasmid

#230993

Purpose

Transient overexpression of mouse FEM1B in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMVQA

Plasmid

#197894

Purpose

pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

Depositor

Thomas Klimkait

Article

Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

Insert

HIV-1 3' end (HXB2: 9045 till poly-A(10)

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RAPH1Δ536-587

Plasmid

#194865

Purpose

Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

RAPH1 with amino acids 536-587 deleted

Promoter

CMV

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-SRO1 JN177

Plasmid

#192546

Purpose

pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)

Use

Gateway Cloning

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 SR3

Plasmid

#192547

Purpose

pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)

Use

Gateway Cloning

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pM-mRuby2

Plasmid

#176550

Purpose

Expression of mRuby2 for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

yomRuby2

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL-mWasabi

Plasmid

#176552

Purpose

Expression of mWasabi for auxotrophic selection in the absence of leucine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

mWasabi

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pM-eCFP

Plasmid

#176558

Purpose

Expression of eCFP for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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