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PlayBack-Ef1a-EcoR1

Plasmid

#203311

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEF6a-CD28-PafA

Plasmid

#113400

Purpose

Expresses CD28-PafA in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

CD28-PafA

Tags

myc

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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FU - dio YFP - W

Plasmid

#73858

Purpose

For expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

eYFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLCS107

Plasmid

#209328

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mNeonGreen fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKL09-GCaMP6f-mScarlet bb118

Plasmid

#158979

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-mScarlet fusion

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc TSC1

Plasmid

#12133

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC1 (TSC1 Human)

Tags

his and myc

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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mEGFP-PLIN3

Plasmid

#222426

Purpose

To express PLIN3 with mEGFP tag in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

PLIN3 (PLIN3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS101

Plasmid

#209355

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mVenus fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA-GW

Plasmid

#209333

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMetYC-Dest

Plasmid

#105081

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

Cub-PLV

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-miRFP703

Plasmid

#113097

Purpose

General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-miRFP703

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Rtn3L-mNeonGreen

Plasmid

#169034

Purpose

Express human Rtn3L in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3L (RTN3 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pME-nfsB-CFP

Plasmid

#86374

Purpose

expression of nfsB-CFP

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

nitroreductase

Use

Zebrafish

Tags

ECFP

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec61β

Plasmid

#172445

Purpose

Expression of ER protein Sec61beta with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-Puro

Plasmid

#207743

Purpose

Expression of the Cre recombinase linked to a puro selectable marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-2A-Puro

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-H2B-mCherry

Plasmid

#135604

Purpose

Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-H2B-GFP

Plasmid

#135605

Purpose

Expression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-GFP

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-P4Mx1

Plasmid

#108143

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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M7 pdnPKA-GFP

Plasmid

#16716

Depositor

Randall Moon

Article

Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

Insert

dnPKA (Prkar1a Mouse)

Use

Xenopus expression

Tags

GFP

Mutation

Dominant negative. Mutations at cAMP binding site…

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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