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Showing: 561 - 580 of 5724 results

  1. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  4. pRB117.2

    Plasmid
    #181949
    Purpose
    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 narL - E. coli, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181949-plasmid-map-sequence-id-356570

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  6. pRB113.2

    Plasmid
    #181948
    Purpose
    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    NarL-mNG; mCherry; tetR (AX04_RS23485, narL narL - E. coli, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181948-plasmid-map-sequence-id-356574

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  7. p2H12ref

    Plasmid
    #221188
    Purpose
    cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    None (same as cdGreen) and Ptet
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221188-plasmid-map-sequence-id-437182

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  8. pRB116.2

    Plasmid
    #181946
    Purpose
    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    TorR-mNG; mCherry; tetR (AX04_RS23485, torR torR - E. coli, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181946-plasmid-map-sequence-id-356600

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  9. pRB120.2

    Plasmid
    #181947
    Purpose
    aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    mNG-TorR; mCherry; tetR (torR, AX04_RS23485 torR - E. coli, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181947-plasmid-map-sequence-id-356558

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  10. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 PhoP-Salmonella enterica subsp. enterica serovar Typhimurium, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181950-plasmid-map-sequence-id-356579

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  13. OVC Core Collection

    Plasmid Kit
    #1000000164
    Purpose
    Three plasmids with the empty cloning cassette in configurations ABC, BAC or ACB, and 21 plasmids containing one of nine light-sensing domains (LSDs) or LSD binding partners.
    Depositor
    Harald Janovjak
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  18. QUAS-0-T7-TetO-GFP_T7-LacO-TetR_ColE1

    Plasmid
    #171663
    Purpose
    QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QUAS-T7-TetO-GFP ssrA-T7 Stop- T7-LacO-TetR-T7 Stop
    Use
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171663-plasmid-map-sequence-id-339028

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  20. R0040 (pTet)_AB

    Plasmid
    #66008
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66008-plasmid-map-sequence-id-114120

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Showing: 561 - 580 of 5724 results