Addgene

We narrowed to 2,387 results for: MEA;

Showing: 561 - 580 of 2387 results

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pFETCh_ZBTB7B

Plasmid

#135730

Purpose

Donor vector for 3' FLAG tag of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B Homology arms (ZBTB7B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry

Plasmid

#68279

Purpose

SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Far1DS NLS mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

RPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pED73 pRS304 pRPS2-SteDS-NLS-mCitrine

Plasmid

#68278

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCitrine

Use

Tags

mCitrine

Expression

Yeast

Mutation

Promoter

pRPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pNB690

Plasmid

#60134

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pFETCh_SOX5

Plasmid

#86292

Purpose

Donor vector for 3' FLAG tag of human SOX5

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SOX5 homology arms (SOX5 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF644

Plasmid

#86264

Purpose

Donor vector for 3' FLAG tag of human ZNF644

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF644 homology arms (ZNF644 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_SOX5_1

Plasmid

#86357

Purpose

Encodes gRNA for 3' target of human SOX5

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SOX5 (SOX5 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

Plasmid

#68274

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pRPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pNB754

Plasmid

#60157

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

LYS9pr

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pNB691

Plasmid

#60135

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB753

Plasmid

#60148

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

LEU1pr

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMA3200

Plasmid

#46855

Purpose

A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

ExoY

Use

Lentiviral

Tags

FKBP E31G, R71G, K105E and Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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Strep-Tev-Avi-hZO-1

Plasmid

#236527

Purpose

For expression of ZO-1 (human) in insect cells

Depositor

Sandra Citi

Article

Spadaro et al Curr Biol. 2017 Dec 18;27(24):3783-3795.

Insert

TJP1 (TJP1 Human)

Use

Tags

SPY Tag and StrepTag-Tev-AviTag

Expression

Insect

Mutation

WT

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Use

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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TMEM63A-W472K-peGFP-N1

Plasmid

#228591

Purpose

Expresses mouse TMEM63A-W472K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Use

Tags

Expression

Mammalian

Mutation

W472K

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM63A-S475K-peGFP-N1

Plasmid

#228592

Purpose

Expresses mouse TMEM63A-S475K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Use

Tags

Expression

Mammalian

Mutation

S475K

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM63A-A476K-peGFP-N1

Plasmid

#228593

Purpose

Expresses mouse TMEM63A-A476K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Use

Tags

Expression

Mammalian

Mutation

A476K

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-AO-66-71-TVA950

Plasmid

#217977

Purpose

AAV Transfer plasmid to express TVA950 in a Cre-dependent manner

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Insert

TVA950

Use

AAV

Tags

Expression

Mutation

Promoter

nEF

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_GpA_G83I

Plasmid

#207844

Purpose

BLaTM-System GpA G83I negative control in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_GpA_wt

Plasmid

#207842

Purpose

BLaTM-System GpA wt positive control in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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