We narrowed to 3,078 results for: RP1

1. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1427E

   Plasmid

   #163998

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R1427E

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1414R

   Plasmid

   #163997

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E1414R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1411R

   Plasmid

   #163996

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E1411R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1397R

   Plasmid

   #163994

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E1397R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E

   Plasmid

   #163993

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R1392E

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E

   Plasmid

   #163992

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R1386E

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Plasmid

   #163059

   Purpose

   mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Orp1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

   Plasmid

   #217321

   Purpose

   Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S969A

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

   Plasmid

   #166814

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1322R

   Promoter

   CMV

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

    Plasmid

    #166811

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    LL1193EE, P1214R, K1277E

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

    Plasmid

    #166812

    Purpose

    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H1276G

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

    Plasmid

    #166813

    Purpose

    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K1277E

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-GFP (F64L, S65T, V163A)-TRP1

    Plasmid

    #53184

    Purpose

    chromosomal tagging with stable GFP

    Depositor

    John Pringle

    Article

    Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (F64L, S65T, V163A)

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CMV-dSa-VPR mini.-2X snRP1

    Plasmid

    #99688

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-5X2-hDrp1(518-736)-Flag

    Plasmid

    #37244

    Depositor

    David Kashatus, Christopher Counter

    Article

    Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

    Insert

    Drp1 (DNM1L Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    contains AA 517-end

    Promoter

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CMV-dSa-VPR mini.-1X snRP1

    Plasmid

    #99687

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Plasmid

    #163075

    Purpose

    Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

    Plasmid

    #166834

    Purpose

    Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Plasmid

    #58442

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Plasmid

    #58440

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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