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pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

Plasmid

#44027

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P405A, P530V, N851A

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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CMVp-dCas9-3xNLS-VP64 (Construct 1)

Plasmid

#55195

Purpose

Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

VP64

Expression

Mammalian

Promoter

CMV/hUBC

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pEMS2113

Plasmid

#49138

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1985

Plasmid

#49116

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

ssAAV-Ple155-iCre

Use

AAV

Expression

Mammalian

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2112

Plasmid

#49137

Purpose

AAV plasmid with NR2E1 (Ple264) promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple264-emGFP

Use

AAV

Expression

Mammalian

Promoter

NR2E1

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2114

Plasmid

#49139

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1990

Plasmid

#49121

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-iCre

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2116

Plasmid

#49141

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

ssAAV-Ple155-emGFP

Use

AAV

Expression

Mammalian

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1981

Plasmid

#49112

Purpose

AAV plasmid with NR2E1 (Ple264) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple264-iCre

Use

AAV

Expression

Mammalian

Promoter

NR2E1

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1920

Plasmid

#82562

Purpose

High copy number plasmid containing Ple253 (PITX3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple253

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1941

Plasmid

#82564

Purpose

High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple264

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1210

Plasmid

#29107

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple238 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1396

Plasmid

#29194

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple146 (NTSR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1174

Plasmid

#29088

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple240 (UGT8 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1617

Plasmid

#29279

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple146 (NTSR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades…

Tags

Intron-LacZ-NLS

Available Since

Oct. 28, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1712

Plasmid

#29297

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple240 (UGT8 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRb delta CDK

Plasmid

#58906

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pEMS1408

Plasmid

#29205

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple201 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1424

Plasmid

#29220

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple155-EGFP-NLS

Use

High copy number homologous recombination plasmid…

Expression

Mammalian

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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