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Showing: 561 - 580 of 623 results

  2. pLZRS-ER-Rac1-Q61L-shortlinker

    Plasmid
    #128584
    Purpose
    For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Glutamine61 to Leucine in the RAC1 moiety.
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128584-plasmid-map-sequence-id-254045

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  3. pLZRS-ER-Rac1-P29S-shortlinker

    Plasmid
    #128583
    Purpose
    For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
    Depositor
    Julian Downward
    Article
    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.
    Insert
    Rac1 (Rac1 Mouse)
    Use
    Retroviral
    Tags
    Modified hormone binding domain of ER alpha
    Expression
    Mammalian
    Mutation
    Changed Proline29 to Serine in the RAC1 moiety.
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128583-plasmid-map-sequence-id-253120

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  11. pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

    Plasmid
    #173963
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif
    Use
    Luciferase
    Tags
    Expression
    Mutation
    1 mutated Coordinator motif
    Promoter
    SV40 promoter
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173963-plasmid-map-sequence-id-341768

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  14. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  17. pGL3-noSV40-EC1.45-human_min1-opossum_min2

    Plasmid
    #173966
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
    Use
    Luciferase
    Tags
    Expression
    Mutation
    See Depositor Comments Below
    Promoter
    SV40 promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173966-plasmid-map-sequence-id-341757

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  18. pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

    Plasmid
    #173994
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated
    Use
    Luciferase
    Tags
    Expression
    Mutation
    7X Coordinator motif mutation #1-2-3-4-5-6-7
    Promoter
    SV40 promoter
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173994-plasmid-map-sequence-id-341795

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  19. pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

    Plasmid
    #173962
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 mutated Coordinator motifs
    Promoter
    SV40 promoter
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173962-plasmid-map-sequence-id-341792

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Showing: 561 - 580 of 623 results