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pHJ8: CadC-VHH-Control

Plasmid

#108243

Purpose

membrane bound transcription activator CadC fusing with anti-RNase A antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Membrane bound transcription activator CadC fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBT29-splitD

Plasmid

#122599

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHJ3: LexA-VHH-Control

Plasmid

#108241

Purpose

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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p416 103Q GAL

Plasmid

#1186

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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FB312

Plasmid

#203569

Purpose

TU for the expression of Nanoluciferase (Nluc) under the PgpdA promoter.

Depositor

Jose Marcos

Article

Gandia et al Microb Biotechnol. 2022 Feb;15(2):630-64

Insert

PgpdA:Nluc:Ttub

Use

Synthetic Biology

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBT137-splitC

Plasmid

#122605

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT137-splitD

Plasmid

#122606

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pFP.E227

Plasmid

#138249

Purpose

Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

mCherry
Terminator
mTagBFP
Terminator
gfp
Terminator

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P17_up1691, P20_992, and iP16_3622

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBT120b

Plasmid

#122604

Purpose

BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

gIII, luxAB, guide RNA

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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ABE8r-V106W

Plasmid

#208295

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-V106W-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET-45b-mCherry

Plasmid

#145279

Purpose

Bacterial Expression of 6xHis mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMK462 (Hygro-P2A-mAB)

Plasmid

#214388

Purpose

mAID-BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Hygro-P2A-mAID-BromoTag

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pNZ18-CAH

Plasmid

#203159

Purpose

Replicates in E. coli, S. cerevisiae, and A. laidlawii

Depositor

Bogumil Karas

Article

Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

Insert

Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCpf1b-sp

Plasmid

#122188

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-RLuc

Plasmid

#188312

Purpose

Expression of Renilla luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Renilla luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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ABE8r-R153del

Plasmid

#208296

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-R153del-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBT44c

Plasmid

#122600

Purpose

BE PACE selection, express C-intein-Cas9-ugi fusion

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

intein-dCas9-ugi fusion

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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