We narrowed to 9,172 results for: mel

1. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

   Plasmid

   #196976

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST

   Depositor

   Lukas Kapitein

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   GST and SNAP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Opie2-PuroR[dsxM]

   Plasmid

   #131616

   Purpose

   Expresses a functional PuroR only in males and dsRed in both genders.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR[dsxM] (dsx Fly)
   dsRed
   

   Expression

   Insect

   Promoter

   Opie2 and hr5Ie1

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGAL4DBD-SuUR f.l

   Plasmid

   #112995

   Purpose

   P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

   Insert

   SuUR (SuUR Fly)
   

   Use

   P insertion vector

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. RP132 pUAST EGFP:Or43a

   Plasmid

   #59523

   Purpose

   N-terminal fusion of EGFP to Drosophila melanogaster Or43a

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   EGFP:Or43a (Or43a Fly)
   

   Tags

   EGFP

   Expression

   Insect

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. KHBD00610

   Plasmid

   #39687

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG31169 (CG43342 Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

6. KHBD00621

   Plasmid

   #39693

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG31392 (CG42726 Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pMesh1*

   Plasmid

   #175591

   Purpose

   Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Mesh1 (Mesh1 Fly)
   

   Tags

   Fused with a flexible GS linker to cerulean

   Expression

   Bacterial

   Mutation

   Codon optimized for translation in E.coli. AGGAGG…

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFD3-ovoD1

   Plasmid

   #111142

   Purpose

   Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

   Insert

   pCFD3-ovoD1 (ovo Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTripz Casp1-IRES-mCherry

   Plasmid

   #187294

   Purpose

   This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

   Depositor

   Alpha Yap

   Article

   Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

   Insert

   Caspase1 (Casp1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   CMV

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis(TEV)-dDrp1-StrepII

    Plasmid

    #174421

    Purpose

    Expresses D. melanogaster Drp1 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1 (Drp1 Fly)
    

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hy_pMT Flag-Hel25E WT

    Plasmid

    #110123

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Oct. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFOSGE_DUM1H01

    Plasmid

    #71090

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    nej (nej Fly)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pGex4t2 with 244-458AA SUUR fragment

    Plasmid

    #112989

    Purpose

    Expresses fusion of GST and amino acids 244-458 of Drosophila SUUR

    Depositor

    Terry Orr-Weaver

    Article

    Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

    Insert

    SuUR (SuUR Fly)
    

    Expression

    Bacterial

    Mutation

    amino acids 244-458

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. KHBD00717

    Plasmid

    #39745

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12124 (mxc Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

15. KHBD00327

    Plasmid

    #34450

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG18783 (Kr-h1 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pGEX6P-1 BICC FL

    Plasmid

    #112998

    Purpose

    For protein expression and purification of full-length Drosophila BicC

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length BicC (BicC Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDmAct5C::Cas9-2A-Neo

    Plasmid

    #176679

    Purpose

    Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    D. melanogaster Act5C

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-white[coffee]

    Plasmid

    #84006

    Purpose

    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

    Depositor

    Phillip Zamore

    Article

    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

    Insert

    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    

    Use

    Unspecified

    Mutation

    A GC-to-AA mutation that creates a G589E missense…

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. KHBD00476

    Plasmid

    #39585

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5838 (Dref Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Hy_pMT Flag-Hel25E K91N

    Plasmid

    #110121

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    K91N mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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