Addgene

We narrowed to 15,032 results for: pho

Showing: 561 - 580 of 15032 results

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pET20b- hTPI

Plasmid

#50723

Purpose

overexpression of human TPI1 in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

6xHIS tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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SPS-7942

Plasmid

#80459

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942

Depositor

Daniel Ducat

Article

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI

Plasmid

#50719

Purpose

expression of human TPI1 in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP2

Plasmid

#51885

Purpose

expression of Xl-VSP2 in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP2

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI Lys13Arg

Plasmid

#50726

Purpose

overexpression of the human TPI1 Lys13Arg allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Lysine 13 to Argenine

Promoter

T7

Available since

Feb. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP1

Plasmid

#51880

Purpose

expression of Xl-VSP1 in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Ile170Val

Plasmid

#50720

Purpose

expression of the human TPI Ile170Val allele in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

Expression

Yeast

Mutation

Isoleucine 170 to Valine

Promoter

GPD

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI Ile170Val

Plasmid

#50724

Purpose

overexpression of the human TPI1 Ile170Val allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Isoleucine 170 to Valine

Promoter

T7

Available since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Lys13Arg

Plasmid

#50722

Purpose

expression of the human TPI Lys13Arg allele in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

Expression

Yeast

Mutation

changed Lysine 13 to Argenine

Promoter

GPD

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Ile170Thr

Plasmid

#50721

Purpose

expression of the human TPI Ile170Thr allele in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

Expression

Yeast

Mutation

Isoleucine 170 to Threonine

Promoter

GPD

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP2 GFP

Plasmid

#51886

Purpose

expression of Xl-VSP2-GFP fusion in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP2

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI Ile170Thr

Plasmid

#50725

Purpose

overexpression of the human TPI1 Ile170Thr allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Isoleucine 170 to Threonine

Promoter

T7

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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ninaE-stops m3-myc

Plasmid

#26855

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

Slow Termination of Phototransduction (stops Fly)

Use

Tags

myc

Expression

Insect

Mutation

Amino acids 296-299 (SLRH in wild-type) are mutat…

Promoter

Available since

April 5, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3-Xl-VSP1-GFP

Plasmid

#51883

Purpose

expression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP1 C301S

Plasmid

#51882

Purpose

expression of Xl-VSP1 inactive mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 C301S (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

C301S, catalytically-inactive mutant of Xl-VSP1

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET28_LmFPPS

Plasmid

#186322

Purpose

pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

Insert

FPPS

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Liskin Swint-Kruse, Emile Van Schaftingen

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-5ptaseOCRL

Plasmid

#66836

Purpose

Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

5ptaseOCRL (OCRL Human)

Use

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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PITPbeta-GFP

Plasmid

#58274

Purpose

mammalian expression of GFP-PITP beta

Depositor

Shamshad Cockcroft

Article

Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

Insert

PITP beta (PITPNB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
CMV-tdTomato-P2A-paCaMKII(T286A)

Plasmid

#165432

Purpose

Expresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII(T286A)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

T286A: Autophosphorylation deficient mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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