We narrowed to 17,542 results for: EMB

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Expression

Bacterial

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Top-T~P70a-Bottom-T

Plasmid

#87314

Purpose

Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMK448 (BromoTag-BSD)

Plasmid

#214378

Purpose

BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BromoTag-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Sox9

Plasmid

#41080

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xHA-Puro

Plasmid

#207729

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST14-DoubleCatcher

Plasmid

#216284

Purpose

Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

DoubleCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pFUS_B2

Plasmid

#31019

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pTN14

Plasmid

#178945

Purpose

Expresses AtTIR1(F79G) in C elegans

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

AtTIR1(F74G)

Expression

Worm

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-Zeo

Plasmid

#207733

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRc/CMV HA TrkB

Plasmid

#39978

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkB (Ntrk2 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3L

Plasmid

#35523

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3L (DNMT3L Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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pME-nfsB-CFP

Plasmid

#86374

Purpose

expression of nfsB-CFP

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

nitroreductase

Use

Zebrafish

Tags

ECFP

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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Reb_206 aka PAS629

Plasmid

#71234

Purpose

Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

mNeonGreen to second copy RebB, soluble mCherry a…

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSV2 neuNT

Plasmid

#10918

Depositor

Bob Weinberg

Article

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuNT (Erbb2 Rat)

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only
phNFL

Plasmid

#132589

Purpose

Expresses human NFL

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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IL-4 promoter luciferase

Plasmid

#12195

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-4 promoter (Il4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miniTurbo-Puro

Plasmid

#207725

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-KO-Puro

Plasmid

#227262

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2_8xCTS-ECFP

Plasmid

#200249

Purpose

Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

8xCTS-ECFP

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc TSC1

Plasmid

#12133

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC1 (TSC1 Human)

Tags

his and myc

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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