We narrowed to 17,428 results for: EMB

1. qTAG-N-Blast-miRFP670nano3

   Plasmid

   #207680

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMetYC-Dest

   Plasmid

   #105081

   Purpose

   Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

   Depositor

   Christopher Grefen

   Article

   Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

   Type

   Empty backbone

   Tags

   Cub-PLV

   Expression

   Yeast

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-CFP

   Plasmid

   #25998

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-CFP (H2BC21 A. victoria, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6.3>gRNA.f+e

   Plasmid

   #99139

   Purpose

   Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   gRNA
   

   Use

   CRISPR

   Promoter

   chicken U6.3

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEnt L1L3 EF1a-tTA-2

   Plasmid

   #26803

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   Elongation Factor 1 alpha promoter and tTA transgene
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMU-GAr

   Plasmid

   #61172

   Purpose

   Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   GmMAN49-mCherry
   

   Expression

   Plant

   Promoter

   AtUBQ10

   Available Since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. LgBiT-ST

   Plasmid

   #207633

   Purpose

   A plasmid encoding LgBiT fused to SpyTag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   LgBiT-SpyTag
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Reb_206 aka PAS629

   Plasmid

   #71234

   Purpose

   Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   mNeonGreen to second copy RebB, soluble mCherry a…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-ultraID-Blast

   Plasmid

   #207715

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pSV2 neuNT

    Plasmid

    #10918

    Depositor

    Bob Weinberg

    Article

    Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

    Insert

    neuNT (Erbb2 Rat)
    

    Expression

    Mammalian

    Available Since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pEJS2699-CMV-Nme2Cas9-E932R

    Plasmid

    #228720

    Purpose

    Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-E932R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS2696-CMV-Nme2SmuCas9-D56R

    Plasmid

    #228717

    Purpose

    Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-D56R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS2697-CMV-Nme2SmuCas9-E932R

    Plasmid

    #228718

    Purpose

    Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-E932R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS2698-CMV-Nme2Cas9-D56R

    Plasmid

    #228719

    Purpose

    Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-D56R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST/LIfeAct-mCherry-N1

    Plasmid

    #40908

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    LifeAct
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    No stop codon to create c-terminal fusion

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKL10-mRuby-iATPSnFR1.0 bb118

    Plasmid

    #158980

    Purpose

    Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mruby-iATPSNfR1.0
    

    Tags

    mruby3

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#102557

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGFP-Rab8A[Q67L]

    Plasmid

    #24900

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A[Q67L]
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Glutamine 67 to Leucine

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP-Rab8A[T22N]

    Plasmid

    #24899

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A[T22N]
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 22 to Asparagine

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYS16

    Plasmid

    #202082

    Purpose

    Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    CT mNG-T2A-PuroR Minicircle Parent
    

    Use

    CRISPR

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-C1-AKT-PH

    Plasmid

    #21218

    Depositor

    Tobias Meyer

    Article

    Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

    Insert

    AKT-PH
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of AKT. (Lab plasmid WC 0002).

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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