We narrowed to 9,277 results for: Pol;

1. pCellFree_G03 EHD2

   Plasmid

   #67107

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   EHD2 (EHD2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pmCherry_C1 I3(resist)

   Plasmid

   #61399

   Purpose

   Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   Inhibitor-3 (PPP1R11 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutations of bases C183T T192C T189C

   Promoter

   CMV

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5FRT/TO-GFP-I3(resist)

   Plasmid

   #61400

   Purpose

   Expresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   Inhibitor-3 (PPP1R11 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   silent mutations of bases C183T T192C T189C

   Promoter

   CMV

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5FRT/TO-GFP-I3(V41A/W43A)

   Plasmid

   #61402

   Purpose

   Expresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   inhibitor-3 (PPP1R11 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   V41A/W43A

   Promoter

   CMV

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. psicheck-2-Mc2r

   Plasmid

   #48174

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of MC2R (MC2R Human)
   

   Use

   Luciferase

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pFM038

   Plasmid

   #40141

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   R183-5A + 7SA; base pairs 6-523 of par-2 were re…

   Promoter

   pie-1

   Available Since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pFM041

   Plasmid

   #40144

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (S241A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   S241A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   pie-1

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pFM039

   Plasmid

   #40142

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   R183-5A + S241A; base pairs 6-523 of par-2 were …

   Promoter

   pie-1

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pFM036

   Plasmid

   #40139

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-2 RNAi resistant (K162A) (par-2 Nematode)
   

   Use

   Gateway destination vector

   Tags

   GFP

   Expression

   Worm

   Mutation

   K162A; base pairs 6-523 of par-2 were recoded to…

   Promoter

   pie-1

   Available Since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pFM052

    Plasmid

    #40146

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (C56S) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    C56S; base pairs 6-523 of par-2 were recoded to …

    Promoter

    pie-1

    Available Since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pFM037

    Plasmid

    #40140

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (R163A) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    R163A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    pie-1

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pLV-hTERT-IRES-hygro

    Plasmid

    #85140

    Purpose

    Lentiviral expression of hTERT, used to create immortalized cell lines

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW mCherry-GFP-LC3

    Plasmid

    #110060

    Purpose

    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

    Depositor

    Anne Brunet

    Article

    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

    Insert

    mCherry-GFP-LC3
    

    Use

    Lentiviral

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTMiniTrex-mCherry-Shark3

    Plasmid

    #233145

    Purpose

    Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark3
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Mutation

    Codon optimized for Trypanosoma cruzi and changed…

    Available Since

    March 20, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GfaABC1D BARK D110A p2A mCherry

    Plasmid

    #117692

    Purpose

    GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs D110A peptide
    

    Use

    AAV

    Tags

    HA / FLAG

    Mutation

    D110A

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7opt in pCAGGS

    Plasmid

    #65974

    Purpose

    Human codon-optimized T7 polymerase

    Depositor

    Benhur Lee

    Article

    Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

    Insert

    T7-opt
    

    Expression

    Mammalian

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. LLP339 pGK-dCas9-SunTag

    Plasmid

    #100943

    Purpose

    dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Src-Y530F

    Plasmid

    #124659

    Purpose

    Human c-Src with Y530F mutation

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    cSrc (SRC Human)
    

    Expression

    Mammalian

    Mutation

    Y530F

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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