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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,359 results for: cas9

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Showing: 5801 - 5820 of 14359 results

  4. pJAT32

    Plasmid
    #204297
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204297-plasmid-map-sequence-id-405508

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  5. pGMC00018 (aka pRC0082)

    Plasmid
    #195320
    Purpose
    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial and Mammalian
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    195320-plasmid-map-sequence-id-387102

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  6. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  10. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

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  11. pCAG-i53-bpA-EF1BFP

    Plasmid
    #111145
    Purpose
    Expression of i53 and a BFP reporter in mammalian cells
    Depositor
    Ralf Kuehn
    Publication
    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    i53 (mutL Synthetic)
    BFP (BFP Synthetic)
    Expression
    Mammalian
    Mutation
    human Ubiquitin, contains 8 point mutations
    Promoter
    CAG promoter and EF1
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111145-plasmid-map-sequence-id-213814

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  16. SK-YFP-SPNM-B

    Plasmid
    #48661
    Purpose
    Bacterial SP and NM repression YFP reporter: protospacer B
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SP/NM prototspacer B/PAM with reporter EYFP
    Use
    CRISPR
    Promoter
    lambda pR promoter
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48661-plasmid-map-sequence-id-71736

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  19. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Available since
    Oct. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    68709-plasmid-map-sequence-id-125636

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Showing: 5801 - 5820 of 14359 results